Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials.
COVID-19
SARS-CoV-2
antigen test
spike protein
thio-NAD cycling
ultrasensitive ELISA
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
25 Oct 2021
25 Oct 2021
Historique:
received:
11
09
2021
revised:
03
10
2021
accepted:
22
10
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
28
11
2021
Statut:
epublish
Résumé
To help control the global pandemic of coronavirus disease 2019 (COVID-19), we developed a diagnostic method targeting the spike protein of the virus that causes the infection, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We applied an ultrasensitive method by combining a sandwich enzyme-linked immunosorbent assay (ELISA) and the thio-nicotinamide adenine dinucleotide (thio-NAD) cycling reaction to quantify spike S1 proteins. The limit of detection (LOD) was 2.62 × 10
Identifiants
pubmed: 34835340
pii: microorganisms9112214
doi: 10.3390/microorganisms9112214
pmc: PMC8619787
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Waseda University
ID : Grant-in-Aid for Young Scientists (Early Bird)
Organisme : Osaka University
ID : Grant for Joint Research Project of the Research Institute for Microbial Diseases
Organisme : Japan Science and Technology Agency
ID : AS3015096U and JPMJTM20LW
Organisme : Japan Society for the Promotion of Science
ID : 20H04556
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