Biochanin A as an α-hemolysin inhibitor for combating methicillin-resistant Staphylococcus aureus infection.
A549 Cells
Animals
Anti-Bacterial Agents
/ administration & dosage
Bacterial Proteins
/ genetics
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Gene Expression Regulation, Bacterial
/ drug effects
Genistein
/ administration & dosage
Hemolysin Proteins
/ genetics
Hemolysis
/ drug effects
Humans
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Mice
Pneumonia
/ drug therapy
Staphylococcal Infections
/ drug therapy
Antivirulence
Biochanin A
Methicillin-resistant Staphylococcus aureus
α-Hemolysin
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
27 Nov 2021
27 Nov 2021
Historique:
received:
09
09
2021
accepted:
28
10
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
5
1
2022
Statut:
epublish
Résumé
Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant pathogen that poses a significant risk to global health today. In S. aureus, α-hemolysin is an important virulence factor as it contributes to the capacity of the bacteria to infect the host. Here, we showed that biochanin A (bioA), an isoflavone present in red clover, cabbage and alfalfa, effectively inhibited hemolytic activity at a dose as low as 32 μg/mL. Further, western blot and RT-qPCR data showed that bioA reduced the production and expression of MRSA hemolysin in a dose-dependent manner. In addition, when different concentrations of bioA were added to a coculture system of A549 cells and S. aureus, it could significantly decrease cell injury. Importantly, the in vivo study showed that bioA could protect mice from pneumonia caused by a lethal dose of MRSA, as evidenced by improving their survival and reducing the number of bacterial colonies in lung tissues, the secretion of hemolysin into alveolar lavage fluid and the degree of pulmonary edema. In conclusion, biochanin A protected the host from MRSA infection by inhibiting the expression of the hemolysin of MRSA, which may provide experimental evidence for its development to a potential anti-MRSA drug.
Identifiants
pubmed: 34837116
doi: 10.1007/s11274-021-03182-4
pii: 10.1007/s11274-021-03182-4
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Hemolysin Proteins
0
Genistein
DH2M523P0H
biochanin A
U13J6U390T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6Subventions
Organisme : Xinglin Scholar Project" of Changchun University of Chinese Medicine
ID : 2019
Organisme : Science and Technology Development Plan Project (2019) of Jilin Province Science and Technology Department
ID : 20190103080JH
Organisme : "Thirteenth Five-Year Plan" of Science and Technology Project of Education Department of Jilin Province
ID : No. JJKH20200906KJ
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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