Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay.
detection time
infectious virus
natural product
quantification
virucidal action
Journal
Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
01
11
2021
received:
20
08
2021
accepted:
09
11
2021
pubmed:
28
11
2021
medline:
29
1
2022
entrez:
27
11
2021
Statut:
ppublish
Résumé
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. The plaques were capable of detecting the virus within 36-40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.
Sections du résumé
BACKGROUND
BACKGROUND
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical.
METHODS
METHODS
Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium.
RESULTS
RESULTS
The plaques were capable of detecting the virus within 36-40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner.
CONCLUSIONS
CONCLUSIONS
These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.
Identifiants
pubmed: 34837712
doi: 10.1002/jcla.24146
pmc: PMC8761427
doi:
Substances chimiques
Antiviral Agents
0
Glycerides
0
monogalactosyl diacylglyceride
0
Galactose
X2RN3Q8DNE
Banques de données
RefSeq
['MN908947.3']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e24146Subventions
Organisme : JST Adaptable and Seamless Technology Transfer Program through Target-driven R & D
ID : JPMJTR204H
Informations de copyright
© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
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