NIR irradiation enhances the apoptotic potentiality of quinacrine-gold hybrid nanoparticles by modulation of HSP-70 in oral cancer stem cells.
Apoptosis
DISC
HSP-70
NIR
OSCC-CSCs
QAuNP
TRAIL
Journal
Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
06
09
2021
revised:
20
10
2021
accepted:
10
11
2021
pubmed:
30
11
2021
medline:
8
4
2022
entrez:
29
11
2021
Statut:
ppublish
Résumé
Cancer stem cells (CSCs) are the tumor cell subpopulations that can self-renew, differentiate, initiate and maintain tumor growth. CSCs are frequently drug-resistant, resulting in tumor recurrence, metastasis, and angiogenesis. Herein, using in vitro oral squamous cell carcinoma (OSCC) CSCs and in vivo xenograft mice model, we have systematically studied the apoptotic potentiality of quinacrine-gold hybrid nanoparticle (QAuNP) and its underlying mechanism after NIR irradiation. QAuNP + NIR caused DNA damage and induced apoptosis in SCC-9-CSCs by deregulating mitochondrial membrane potential (ΔΨm) and activation of ROS. Upregulation of CASPASE-3 and DR-5/DR-4 and reduction of heat shock protein (HSP-70) up to 5-fold were also noticed upon the treatment. The increased expression of DR-5 and CASPASE-3 and decreased expression of HSP-70, CD-44 and Ki-67 were also noted in the xenograft mice treated with QAuNP + NIR + TRAIL. Thus, data suggest that the combined treatment enhances apoptosis in OSCC-CSCs by modulating HSP-70 in the DISC.
Identifiants
pubmed: 34843984
pii: S1549-9634(21)00145-3
doi: 10.1016/j.nano.2021.102502
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Gold
7440-57-5
Quinacrine
H0C805XYDE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102502Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.