Galectin network in osteoarthritis: galectin-4 programs a pathogenic signature of gene and effector expression in human chondrocytes in vitro.
3D spheres
Cartilage
Inflammation
Lectin
NF-κB
Primary cells
Journal
Histochemistry and cell biology
ISSN: 1432-119X
Titre abrégé: Histochem Cell Biol
Pays: Germany
ID NLM: 9506663
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
accepted:
08
11
2021
pubmed:
1
12
2021
medline:
15
3
2022
entrez:
30
11
2021
Statut:
ppublish
Résumé
Galectin-4 (Gal-4) is a member of the galectin family, which have been identified as galactose-binding proteins. Gal-4 possesses two tandem repeat carbohydrate recognition domains and acts as a cross-linking bridge in sulfatide-dependent glycoprotein routing. We herein document its upregulation in osteoarthritis (OA) in correlation with the extent of cartilage degradation in vivo. Primary human OA chondrocytes in vitro respond to carbohydrate-inhibitable Gal-4 binding with the upregulation of pro-degradative/-inflammatory proteins such as interleukin-1β (IL-1β) and matrix metalloproteinase-13 (MMP-13), as documented by RT-qPCR-based mRNA profiling and transcriptome data processing. Activation of p65 by phosphorylation of Ser536 within the NF-κB pathway and the effect of three p65 inhibitors on Gal-4 activity support downstream involvement of such signaling. In 3D (pellet) cultures, Gal-4 presence causes morphological and biochemical signs of degradation. Taken together, our findings strongly support the concept of galectins acting as a network in OA pathogenesis and suggest that blocking their activity in disease progression may become clinically relevant in the future.
Identifiants
pubmed: 34846578
doi: 10.1007/s00418-021-02053-1
pii: 10.1007/s00418-021-02053-1
pmc: PMC8847242
doi:
Substances chimiques
Galectin 4
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
139-151Informations de copyright
© 2021. The Author(s).
Références
Cell Mol Biol (Noisy-le-grand). 1999 Dec;45(8):1191-202
pubmed: 10643968
J Orthop Res. 2019 Oct;37(10):2204-2216
pubmed: 31115931
Immunol Rev. 1998 Jun;163:19-34
pubmed: 9700499
Arthritis Res Ther. 2013 Jan 08;15(1):R5
pubmed: 23298463
Blood. 2007 Jan 1;109(1):219-27
pubmed: 16940423
iScience. 2020 Dec 10;24(1):101919
pubmed: 33409472
Sci Rep. 2020 Sep 24;10(1):15708
pubmed: 32973213
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
J Biol Chem. 1993 Mar 15;268(8):5929-39
pubmed: 8449956
Biochim Biophys Acta. 2002 Sep 19;1572(2-3):209-31
pubmed: 12223271
Arthritis Res Ther. 2007;9(1):R20
pubmed: 17326835
Dev Biol. 2001 Jan 1;229(1):203-14
pubmed: 11133164
Cancer Res. 2007 Jul 1;67(13):6155-62
pubmed: 17616672
Histochem Cell Biol. 2017 Feb;147(2):239-256
pubmed: 28012132
Anat Rec (Hoboken). 2015 Dec;298(12):2051-70
pubmed: 26340709
Cell Death Differ. 2011 May;18(5):806-16
pubmed: 21113146
Blood. 2006 Oct 1;108(7):2399-406
pubmed: 16778138
Histochem Cell Biol. 2014 Oct;142(4):373-88
pubmed: 24981556
Dev Biol. 1982 Aug;92(2):544-8
pubmed: 7117700
J Biol Chem. 2005 Nov 4;280(44):37266-77
pubmed: 16105842
Histochem Cell Biol. 2017 Feb;147(2):111-117
pubmed: 27975142
Curr Opin Rheumatol. 2004 Sep;16(5):595-8
pubmed: 15314500
J Exp Med. 2012 Dec 17;209(13):2383-94
pubmed: 23209314
Proteomics Clin Appl. 2021 May;15(2-3):e2000057
pubmed: 33580901
Bioinformatics. 2004 Feb 12;20(3):307-15
pubmed: 14960456
Biochem Biophys Res Commun. 2009 Jan 30;379(1):155-9
pubmed: 19101505
Chem Asian J. 2013 Jul;8(7):1536-50
pubmed: 23640760
Nucleic Acids Res. 2015 Apr 20;43(7):e47
pubmed: 25605792
IUBMB Life. 2019 Mar;71(3):364-375
pubmed: 30550624
J Cell Biol. 2005 May 9;169(3):491-501
pubmed: 15883199
Tumour Biol. 2017 Mar;39(3):1010428317691687
pubmed: 28345468
J Proteome Res. 2016 Dec 2;15(12):4412-4422
pubmed: 27801591
BMC Dev Biol. 2011 Feb 01;11:6
pubmed: 21284876
F1000Res. 2014 Jul 01;3:141
pubmed: 25075306
Int J Mol Sci. 2021 Feb 09;22(4):
pubmed: 33572320
J Bone Joint Surg Am. 1971 Apr;53(3):523-37
pubmed: 5580011
FEBS J. 2007 Jul;274(13):3233-56
pubmed: 17535296
J Cell Biol. 1992 Dec;119(6):1689-700
pubmed: 1469056
J Biol Chem. 2001 Sep 21;276(38):35917-23
pubmed: 11451961
Dev Dyn. 1996 Jul;206(3):260-71
pubmed: 8896982
Glycobiology. 2003 Oct;13(10):713-23
pubmed: 12851289
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Chem Rev. 1998 Apr 2;98(2):637-674
pubmed: 11848911
J Immunol. 2009 Apr 1;182(7):4036-45
pubmed: 19299701
J Biol Chem. 1994 Dec 16;269(50):31770-6
pubmed: 7989350
Histopathology. 2007 Nov;51(5):681-90
pubmed: 17927590
J Neurochem. 2013 Apr;125(1):49-62
pubmed: 23311731
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48
pubmed: 34012175
Int J Cancer. 2011 Aug 15;129(4):799-809
pubmed: 21064109
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):E2509-E2518
pubmed: 29382751
Ann Rheum Dis. 2008 Feb;67(2):175-81
pubmed: 17545192
J Biol Chem. 2010 Jan 22;285(4):2232-44
pubmed: 19920154
Biochem J. 2019 Sep 24;476(18):2623-2655
pubmed: 31551311
J Immunol. 2016 Feb 15;196(4):1910-21
pubmed: 26792806
Biochim Biophys Acta. 2002 Sep 19;1572(2-3):232-54
pubmed: 12223272
Sci Rep. 2016 Dec 16;6:39112
pubmed: 27982117
J Biol Chem. 2005 Feb 11;280(6):4730-7
pubmed: 15546874
Immunity. 2004 Jun;20(6):681-93
pubmed: 15189734
Cell Mol Life Sci. 2018 Nov;75(22):4187-4205
pubmed: 29934665
Arthritis Res Ther. 2013 Oct 04;15(5):R147
pubmed: 24289744
Histochem Cell Biol. 2017 Feb;147(2):223-237
pubmed: 27999992
FASEB J. 2018 Jun 15;:fj201800687
pubmed: 29906246
Chemistry. 2013 Feb 4;19(6):1896-902
pubmed: 23280962
FEBS J. 2012 Nov;279(21):4062-80
pubmed: 22943525
Calcif Tissue Int. 2021 Mar;108(3):377-390
pubmed: 33185768
BMC Mol Biol. 2010 Sep 21;11:74
pubmed: 20858237