Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus.
Biomedical Engineering
/ methods
Blood Urea Nitrogen
Diagnostic Tests, Routine
Early Diagnosis
Genotype
Hepacivirus
Hepatitis C
/ diagnosis
Humans
Laboratories
Microfluidics
/ methods
Molecular Diagnostic Techniques
/ methods
Nucleic Acid Amplification Techniques
/ methods
Point-of-Care Systems
Viral Load
World Health Organization
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 11 2021
30 11 2021
Historique:
received:
29
03
2021
accepted:
27
10
2021
entrez:
1
12
2021
pubmed:
2
12
2021
medline:
4
1
2022
Statut:
epublish
Résumé
The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in <40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is manufactured at minimal cost using simple, portable equipment. Further development of the diagnostic test will facilitate linkage between disease diagnosis and treatment, greatly improving patient care pathways and reducing loss to follow-up, so assisting in the global elimination strategy.
Identifiants
pubmed: 34848705
doi: 10.1038/s41467-021-27076-z
pii: 10.1038/s41467-021-27076-z
pmc: PMC8632961
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6994Subventions
Organisme : Medical Research Council
ID : MC_UU_12014/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0801566
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0901213-92157
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K013491/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16045
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : 102789/Z/13/A
Informations de copyright
© 2021. The Author(s).
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