Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 11 2021
30 11 2021
Historique:
received:
25
02
2021
accepted:
12
11
2021
entrez:
1
12
2021
pubmed:
2
12
2021
medline:
4
1
2022
Statut:
epublish
Résumé
Biocompatible fluorescent reporters with spectral properties spanning the entire visible spectrum are indispensable tools for imaging the biochemistry of living cells and organisms in real time. Here, we report the engineering of a fluorescent chemogenetic reporter with tunable optical and spectral properties. A collection of fluorogenic chromophores with various electronic properties enables to generate bimolecular fluorescent assemblies that cover the visible spectrum from blue to red using a single protein tag engineered and optimized by directed evolution and rational design. The ability to tune the fluorescence color and properties through simple molecular modulation provides a broad experimental versatility for imaging proteins in live cells, including neurons, and in multicellular organisms, and opens avenues for optimizing Förster resonance energy transfer (FRET) biosensors in live cells. The ability to tune the spectral properties and fluorescence performance enables furthermore to match the specifications and requirements of advanced super-resolution imaging techniques.
Identifiants
pubmed: 34848727
doi: 10.1038/s41467-021-27334-0
pii: 10.1038/s41467-021-27334-0
pmc: PMC8633346
doi:
Substances chimiques
Biocompatible Materials
0
Coloring Agents
0
Cyan Fluorescent Protein
0
Fluorescent Dyes
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6989Informations de copyright
© 2021. The Author(s).
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