Inhibitory properties of crude microalgal extracts on the in vitro replication of cyprinid herpesvirus 3.
Animals
Aquaculture
Biomass
Biotechnology
Carps
/ virology
Chlamydomonas reinhardtii
Chlorella
Chlorophyceae
/ genetics
Chlorophyta
Complex Mixtures
Cyanobacteria
/ genetics
DNA Replication
DNA, Viral
Ethanol
Herpesviridae
In Vitro Techniques
Inhibitory Concentration 50
Microalgae
/ metabolism
Nostoc
Scenedesmus
Spirulina
Virus Diseases
/ therapy
Virus Replication
Water Microbiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 11 2021
30 11 2021
Historique:
received:
24
06
2021
accepted:
11
11
2021
entrez:
1
12
2021
pubmed:
2
12
2021
medline:
15
2
2022
Statut:
epublish
Résumé
Microalgae are possible sources of antiviral substances, e.g. against cyprinid herpesvirus 3 (CyHV-3). Although this virus leads to high mortalities in aquacultures, there is no treatment available yet. Hence, ethanolic extracts produced with accelerated solvent extraction from six microalgal species (Arthrospira platensis, Chlamydomonas reinhardtii, Chlorella kessleri, Haematococcus pluvialis, Nostoc punctiforme and Scenedesmus obliquus) were examined in this study. An inhibition of the in vitro replication of CyHV-3 could be confirmed for all six species, with the greatest effect for the C. reinhardtii and H. pluvialis crude extracts. At still non-cytotoxic concentrations, viral DNA replication was reduced by over 3 orders of magnitude each compared to the untreated replication controls, while the virus titers were even below the limit of detection (reduction of 4 orders of magnitude). When pre-incubating both cells and virus with C. reinhardtii and H. pluvialis extracts before inoculation, the reduction of viral DNA was even stronger (> 4 orders of magnitude) and no infectious viral particles were detected. Thus, the results of this study indicate that microalgae and cyanobacteria are a promising source of natural bioactive substances against CyHV-3. However, further studies regarding the isolation and identification of the active components of the extracts are needed.
Identifiants
pubmed: 34848777
doi: 10.1038/s41598-021-02542-2
pii: 10.1038/s41598-021-02542-2
pmc: PMC8633293
doi:
Substances chimiques
Complex Mixtures
0
DNA, Viral
0
Ethanol
3K9958V90M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
23134Informations de copyright
© 2021. The Author(s).
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