Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish.

Animals Antifungal Agents / therapeutic use Disease Models, Animal Epithelial Cells / pathology Larva / microbiology Mycoses / drug therapy Zebrafish / microbiology
Cell Biology Microbiology Model Organisms Molecular Biology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 12 2021
Historique:
entrez: 1 12 2021
pubmed: 2 12 2021
medline: 2 12 2021
Statut: epublish

Résumé

Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species. For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).

Identifiants

DOI: 10.1016/j.xpro.2021.100963 PMID: 34849488 PMC: PMC8609045
pubmed: 34849488
doi: 10.1016/j.xpro.2021.100963
pii: S2666-1667(21)00669-9
pmc: PMC8609045
doi:

Substances chimiques

Antifungal Agents 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100963

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM124043
Pays : United States

Informations de copyright

© 2021 The Authors.

Déclaration de conflit d'intérêts

D.P.K. reports honoraria and research support from Gilead Sciences, received consultant fees from Astellas Pharma and Gilead Sciences, and is a member of the Data Review Committee of Cidara Therapeutics. All other authors declare no competing interests.

Références

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Curr Biol. 2021 Mar 22;31(6):1129-1140.e4
pubmed: 33400921

Auteurs

Sebastian Wurster (S)

Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Oscar E Ruiz (OE)

Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Alexander M Tatara (AM)

Medical Scientist Training Program, Baylor College of Medicine, Houston, TX 77030, USA.

Dimitrios P Kontoyiannis (DP)

Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

George T Eisenhoffer (GT)

Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Genetics and Epigenetics Graduate Program, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Protocol for fungal infection following the...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antifongiques › Protocol for fungal infection following the...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Protocol for fungal infection following the...
questionsmedicales.fr › Cellules › Cellules épithéliales › Protocol for fungal infection following the...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Protocol for fungal infection following the...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Mycoses › Protocol for fungal infection following the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Protocol for fungal infection following the...