Structure and function of axo-axonic inhibition.
axon initial segment
connectomics
inhibition
mouse
neuroscience
visual cortex
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
09
09
2021
accepted:
30
11
2021
pubmed:
2
12
2021
medline:
22
1
2022
entrez:
1
12
2021
Statut:
epublish
Résumé
Inhibitory neurons in mammalian cortex exhibit diverse physiological, morphological, molecular, and connectivity signatures. While considerable work has measured the average connectivity of several interneuron classes, there remains a fundamental lack of understanding of the connectivity distribution of distinct inhibitory cell types with synaptic resolution, how it relates to properties of target cells, and how it affects function. Here, we used large-scale electron microscopy and functional imaging to address these questions for chandelier cells in layer 2/3 of the mouse visual cortex. With dense reconstructions from electron microscopy, we mapped the complete chandelier input onto 153 pyramidal neurons. We found that synapse number is highly variable across the population and is correlated with several structural features of the target neuron. This variability in the number of axo-axonic ChC synapses is higher than the variability seen in perisomatic inhibition. Biophysical simulations show that the observed pattern of axo-axonic inhibition is particularly effective in controlling excitatory output when excitation and inhibition are co-active. Finally, we measured chandelier cell activity in awake animals using a cell-type-specific calcium imaging approach and saw highly correlated activity across chandelier cells. In the same experiments, in vivo chandelier population activity correlated with pupil dilation, a proxy for arousal. Together, these results suggest that chandelier cells provide a circuit-wide signal whose strength is adjusted relative to the properties of target neurons.
Identifiants
pubmed: 34851292
doi: 10.7554/eLife.73783
pii: 73783
pmc: PMC8758143
doi:
pii:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIMH NIH HHS
ID : RF1 MH123400
Pays : United States
Organisme : NINDS NIH HHS
ID : U19 NS104648
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY027036
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH114824
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS104926
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1MH117815
Pays : United States
Informations de copyright
© 2021, Schneider-Mizell et al.
Déclaration de conflit d'intérêts
CS, AB, FC, DB, AB, SD, NT, KL, RL, JW, JZ, AN, BH, JB, MT, RT, GM, DB, YL, TC, NK, WS, DI, JZ, AZ, IT, SP, WW, MC, CJ, EF, LB, SS, CA, RR, NC No competing interests declared, TM discloses financial interests in Zetta AI LLC, JR, AT discloses financial interests in Vathes LLC, HS discloses financial interests in Zetta AI
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