Live attenuated virus vaccine protects against SARS-CoV-2 variants of concern B.1.1.7 (Alpha) and B.1.351 (Beta).


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
03 Dec 2021
Historique:
entrez: 1 12 2021
pubmed: 2 12 2021
medline: 2 12 2021
Statut: ppublish

Résumé

Vaccines are instrumental and indispensable in the fight against the COVID-19 pandemic. Several recent SARS-CoV-2 variants are more transmissible and evade infection- or vaccine-induced protection. We constructed live attenuated vaccine candidates by large-scale recoding of the SARS-CoV-2 genome and showed that the lead candidate, designated sCPD9, protects Syrian hamsters from a challenge with ancestral virus. Here, we assessed immunogenicity and protective efficacy of sCPD9 in the Roborovski dwarf hamster, a nontransgenic rodent species that is highly susceptible to SARS-CoV-2 and severe COVID-19–like disease. We show that a single intranasal vaccination with sCPD9 elicited strong cross-neutralizing antibody responses against four current SARS-CoV-2 variants of concern, B.1.1.7 (Alpha), B.1.351 (Beta), B.1.1.28.1 (Gamma), and B.1.617.2 (Delta). The sCPD9 vaccine offered complete protection from COVID-19–like disease caused by the ancestral SARS-CoV-2 variant B.1 and the two variants of concern B.1.1.7 and B.1.351.

Identifiants

pubmed: 34851677
doi: 10.1126/sciadv.abk0172
pmc: PMC8635430
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabk0172

Références

Science. 2021 Aug 13;373(6556):818-823
pubmed: 34016740
Cell Rep. 2020 Dec 8;33(10):108488
pubmed: 33271063
Nat Med. 2021 May;27(5):917-924
pubmed: 33772244
Nature. 2021 Aug;596(7871):276-280
pubmed: 34237773
Science. 2008 Jun 27;320(5884):1784-7
pubmed: 18583614
Nat Med. 2021 Jul;27(7):1230-1238
pubmed: 34035535
N Engl J Med. 2021 May 20;384(20):1899-1909
pubmed: 33951374
JAMA. 2021 Mar 2;325(9):821-822
pubmed: 33507218
Cell. 2021 May 27;184(11):2939-2954.e9
pubmed: 33852911
PLoS Pathog. 2018 Jan 29;14(1):e1006857
pubmed: 29377958
Nature. 2021 May;593(7857):142-146
pubmed: 33780970
N Engl J Med. 2021 Jul 8;385(2):187-189
pubmed: 33951357
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
J Gen Virol. 2018 Dec;99(12):1705-1716
pubmed: 30113295
Vaccines (Basel). 2021 Apr 07;9(4):
pubmed: 33916924
BMJ. 2021 Aug 2;374:n1933
pubmed: 34340962
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Lancet Reg Health Am. 2021 Sep;1:100025
pubmed: 34386791
Lancet. 2021 May 8;397(10286):1725-1735
pubmed: 33901423
Cell Rep. 2021 Aug 3;36(5):109493
pubmed: 34320400
Science. 2021 Apr 9;372(6538):
pubmed: 33658326
Proc Natl Acad Sci U S A. 1989 May;86(10):3699-703
pubmed: 2657727
Science. 2021 May 21;372(6544):815-821
pubmed: 33853970
Am J Respir Cell Mol Biol. 2020 Dec;63(6):856-859
pubmed: 32897757
Cell. 2021 Aug 5;184(16):4220-4236.e13
pubmed: 34242578
Cell Rep. 2020 Apr 28;31(4):107586
pubmed: 32348767
Nat Med. 2021 Mar;27(3):440-446
pubmed: 33531709
Viruses. 2020 Jul 20;12(7):
pubmed: 32698441
Lancet. 2021 May 15;397(10287):1819-1829
pubmed: 33964222
Nature. 2021 May;593(7858):266-269
pubmed: 33767447
N Engl J Med. 2021 May 20;384(20):1885-1898
pubmed: 33725432
Nature. 2021 May;593(7857):130-135
pubmed: 33684923

Auteurs

Jakob Trimpert (J)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.

Julia M Adler (JM)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.
Department of Infectious Diseases and Respiratory Medicine, Charité, Universitätsmedizin Berlin, Berlin, Germany.

Kathrin Eschke (K)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.

Azza Abdelgawad (A)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.

Theresa C Firsching (TC)

Institut für Tierpathologie, Freie Universität Berlin, Berlin, Germany.

Nadine Ebert (N)

Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Tran Thi Nhu Thao (TTN)

Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.

Achim D Gruber (AD)

Institut für Tierpathologie, Freie Universität Berlin, Berlin, Germany.

Volker Thiel (V)

Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Nikolaus Osterrieder (N)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.
Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong.

Dusan Kunec (D)

Institut für Virologie, Freie Universität Berlin, Berlin, Germany.

Classifications MeSH