Characterization and comparison of recombinant full-length ursine and human sex hormone-binding globulin.
Ursus arctos
hibernation
insect cells
sex hormone-binding globulin
steroid
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
08
11
2021
received:
02
06
2021
accepted:
30
11
2021
pubmed:
3
12
2021
medline:
1
4
2022
entrez:
2
12
2021
Statut:
ppublish
Résumé
Sex hormone-binding globulin (SHBG) regulates the bioavailability of sex steroid hormones in the blood. Levels of SHBG increase markedly in brown bears (Ursus arctos) during hibernation, suggesting that a key regulatory role of this protein is to quench sex steroid bioavailability in hibernation physiology. To enable characterization of ursine SHBG and a cross species comparison, we established an insect cell-based expression system for recombinant full-length ursine and human SHBG. Compared with human SHBG, we observed markedly lower secretion levels of ursine SHBG, resulting in a 10-fold difference in purified protein yield. Both human and ursine recombinant SHBG appeared as dimeric proteins in solution, with a single unfolding temperature of ~ 58 °C. The thermal stability of ursine and human SHBG increased 5.4 and 9.5 °C, respectively, in the presence of dihydrotestosterone (DHT), suggesting a difference in affinity. The dissociation constants for [
Identifiants
pubmed: 34855305
doi: 10.1002/2211-5463.13341
pmc: PMC8804615
doi:
Substances chimiques
Recombinant Proteins
0
Sex Hormone-Binding Globulin
0
Steroids
0
Dihydrotestosterone
08J2K08A3Y
Banques de données
RefSeq
['NP_001031', 'XP_026376673']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
362-378Informations de copyright
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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