Investigation of the Effect of Prunus Amygdalus Amara on the Expression of some Genes of Apoptosis and Immortality in Breast Cancer Cells (MCF- 7).
Bax
Bcl2
Breast cancer
apoptosis
bitter almond
β-actin
Journal
Current drug research reviews
ISSN: 2589-9783
Titre abrégé: Curr Drug Res Rev
Pays: United Arab Emirates
ID NLM: 101735701
Informations de publication
Date de publication:
2022
2022
Historique:
received:
13
07
2021
revised:
16
09
2021
accepted:
11
10
2021
pubmed:
4
12
2021
medline:
18
5
2022
entrez:
3
12
2021
Statut:
ppublish
Résumé
Anti-cancer effects of almond nuts or oil have been approved, but there are a few pieces of research that have evaluated, in detail, almond and other seeds' effects on cancer. Therefore, in the present project, the aim was to explore the regulatory effect of the bitter almond extract (Prunus amygdalus Batsch) on the apoptotic and anti-cancer potency of MCF-7 cells. In the current experimental research, the almond effect on MCF7 cells was evaluated by investigating the expression and the balance between Bcl-2, Bax genes to unmark the potential molecular mechanism. For 24 and 48h, the MCF7 cells were treated with the bitter almond extract (187.5-3000 μg/mL). MTT assay was used to assess the viability, and Real-time-PCR was applied to determine the expression of Bax and Bcl-2, facing β-actin. Our results revealed a significant difference between different extract concentrations on the viability of MCF7 cell lines in 24 and 48 h; cell viability decreased time-dependently (P < 0.05). After 24 and 48h of extract facing MCF7 cells, the evaluated IC50 value was 3000 and 1500 μg/mL, respectively. Based on Real-Time-PCR analysis, after 24 and 48 h, the mRNA levels of BCL-2 decreased by the extract, whereas Bax was in the MCF-7 cell line. From the results, it can be concluded that bitter almond extract has anti-cancer properties that may influence the apoptotic pathways by regulating relative gene expression.
Sections du résumé
BACKGROUND
Anti-cancer effects of almond nuts or oil have been approved, but there are a few pieces of research that have evaluated, in detail, almond and other seeds' effects on cancer. Therefore, in the present project, the aim was to explore the regulatory effect of the bitter almond extract (Prunus amygdalus Batsch) on the apoptotic and anti-cancer potency of MCF-7 cells.
OBJECTIVE
In the current experimental research, the almond effect on MCF7 cells was evaluated by investigating the expression and the balance between Bcl-2, Bax genes to unmark the potential molecular mechanism.
METHODS
For 24 and 48h, the MCF7 cells were treated with the bitter almond extract (187.5-3000 μg/mL). MTT assay was used to assess the viability, and Real-time-PCR was applied to determine the expression of Bax and Bcl-2, facing β-actin.
RESULTS
Our results revealed a significant difference between different extract concentrations on the viability of MCF7 cell lines in 24 and 48 h; cell viability decreased time-dependently (P < 0.05). After 24 and 48h of extract facing MCF7 cells, the evaluated IC50 value was 3000 and 1500 μg/mL, respectively. Based on Real-Time-PCR analysis, after 24 and 48 h, the mRNA levels of BCL-2 decreased by the extract, whereas Bax was in the MCF-7 cell line.
CONCLUSION
From the results, it can be concluded that bitter almond extract has anti-cancer properties that may influence the apoptotic pathways by regulating relative gene expression.
Identifiants
pubmed: 34856918
pii: CDRR-EPUB-119192
doi: 10.2174/2589977513666211202094433
doi:
Substances chimiques
Plant Extracts
0
Proto-Oncogene Proteins c-bcl-2
0
bcl-2-Associated X Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
73-79Informations de copyright
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