Seven-year kinetics of RTS, S/AS01-induced anti-CSP antibodies in young Kenyan children.


Journal

Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802

Informations de publication

Date de publication:
02 Dec 2021
Historique:
received: 31 07 2021
accepted: 20 10 2021
entrez: 3 12 2021
pubmed: 4 12 2021
medline: 15 12 2021
Statut: epublish

Résumé

RTS,S/AS01, the leading malaria vaccine has been recommended by the WHO for widespread immunization of children at risk. RTS,S/AS01-induced anti-CSP IgG antibodies are associated with the vaccine efficacy. Here, the long-term kinetics of RTS,S/AS01-induced antibodies was investigated. 150 participants were randomly selected from the 447 children who participated in the RTS,S/AS01 phase IIb clinical trial in 2007 from Kilifi-Kenya. Cumulatively, the retrospective follow-up period was 93 months with annual plasma samples collection. The levels of anti-CSP IgM, total IgG, IgG1, IgG2, IgG3, and IgG4 antibodies were then determined using an enzyme-linked immunosorbent assay. RTS,S/AS01 induced high levels of anti-CSP IgG antibodies which exhibited a rapid waning over 6.5 months post-vaccination, followed by a slower decay over the subsequent years. RTS,S/AS01-induced anti-CSP IgG antibodies remained elevated above the control group levels throughout the 7 years follow-up period. The anti-CSP IgG antibodies were mostly IgG1, IgG3, IgG2, and to a lesser extent IgG4. IgG2 predominated in later timepoints. RTS,S/AS01 also induced high levels of anti-CSP IgM antibodies which increased above the control group levels by month 3. The controls exhibited increasing levels of the anti-CSP IgM antibodies which caught up with the RTS,S/AS01 vaccinees levels by month 21. In contrast, there were no measurable anti-CSP IgG antibodies among the controls. RTS,S/AS01-induced anti-CSP IgG antibodies kinetics are consistent with long-lived but waning vaccine efficacy. Natural exposure induces anti-CSP IgM antibodies in children, which increases with age, but does not induce substantial levels of anti-CSP IgG antibodies.

Sections du résumé

BACKGROUND BACKGROUND
RTS,S/AS01, the leading malaria vaccine has been recommended by the WHO for widespread immunization of children at risk. RTS,S/AS01-induced anti-CSP IgG antibodies are associated with the vaccine efficacy. Here, the long-term kinetics of RTS,S/AS01-induced antibodies was investigated.
METHODS METHODS
150 participants were randomly selected from the 447 children who participated in the RTS,S/AS01 phase IIb clinical trial in 2007 from Kilifi-Kenya. Cumulatively, the retrospective follow-up period was 93 months with annual plasma samples collection. The levels of anti-CSP IgM, total IgG, IgG1, IgG2, IgG3, and IgG4 antibodies were then determined using an enzyme-linked immunosorbent assay.
RESULTS RESULTS
RTS,S/AS01 induced high levels of anti-CSP IgG antibodies which exhibited a rapid waning over 6.5 months post-vaccination, followed by a slower decay over the subsequent years. RTS,S/AS01-induced anti-CSP IgG antibodies remained elevated above the control group levels throughout the 7 years follow-up period. The anti-CSP IgG antibodies were mostly IgG1, IgG3, IgG2, and to a lesser extent IgG4. IgG2 predominated in later timepoints. RTS,S/AS01 also induced high levels of anti-CSP IgM antibodies which increased above the control group levels by month 3. The controls exhibited increasing levels of the anti-CSP IgM antibodies which caught up with the RTS,S/AS01 vaccinees levels by month 21. In contrast, there were no measurable anti-CSP IgG antibodies among the controls.
CONCLUSION CONCLUSIONS
RTS,S/AS01-induced anti-CSP IgG antibodies kinetics are consistent with long-lived but waning vaccine efficacy. Natural exposure induces anti-CSP IgM antibodies in children, which increases with age, but does not induce substantial levels of anti-CSP IgG antibodies.

Identifiants

pubmed: 34856981
doi: 10.1186/s12936-021-03961-2
pii: 10.1186/s12936-021-03961-2
pmc: PMC8641151
doi:

Substances chimiques

Antibodies, Protozoan 0
Malaria Vaccines 0
Protozoan Proteins 0
circumsporozoite protein, Protozoan 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

452

Subventions

Organisme : Medical Research Council
ID : MR/P020321/1
Pays : United Kingdom
Organisme : european and developing countries clinical trials partnership
ID : TMA2016SF-1513

Informations de copyright

© 2021. The Author(s).

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Auteurs

Robert M Mugo (RM)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya. Robert.mugo@fu-berlin.de.
Institute of Immunology, Center for Infection Medicine, Freie Universtät Berlin, 14163, Berlin, Germany. Robert.mugo@fu-berlin.de.
Department of Biological Sciences, Pwani University, P.O. Box 195-80108, Kilifi, Kenya. Robert.mugo@fu-berlin.de.

Kennedy Mwai (K)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.

Jedidah Mwacharo (J)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.

Faiz M Shee (FM)

Department of Biological Sciences, Pwani University, P.O. Box 195-80108, Kilifi, Kenya.

Jennifer N Musyoki (JN)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.

Juliana Wambua (J)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.

Edward Otieno (E)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.

Philip Bejon (P)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Francis M Ndungu (FM)

KEMRI-Wellcome Trust Collaborative Programme, P.O. Box 230, Kilifi, 80108, Kenya. FNdungu@kemri-wellcome.org.
Institute of Immunology, Center for Infection Medicine, Freie Universtät Berlin, 14163, Berlin, Germany. FNdungu@kemri-wellcome.org.
Department of Biological Sciences, Pwani University, P.O. Box 195-80108, Kilifi, Kenya. FNdungu@kemri-wellcome.org.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK. FNdungu@kemri-wellcome.org.
Division of Infectious Diseases, Department of Medicine Solna and Centre for Molecular Medicine, Karolinska Institute, Stockholm, Sweden. FNdungu@kemri-wellcome.org.

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Classifications MeSH