Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on
FTIR spectroscopy
Phaeodactylum tricornutum Bohlin
algal physiology
lipid droplets
macromolecules quantification
phytoplankton
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2021
2021
Historique:
received:
10
08
2021
accepted:
07
09
2021
entrez:
3
12
2021
pubmed:
4
12
2021
medline:
4
12
2021
Statut:
epublish
Résumé
Diatoms are feedstock for the production of sustainable biocommodities, including biofuel. The biochemical characterization of newly isolated or genetically modified strains is seminal to identify the strains that display interesting features for both research and industrial applications. Biochemical quantification of organic macromolecules cellular quotas are time-consuming methodologies which often require large amount of biological sample. Vibrational spectroscopy is an essential tool applied in several fields of research. A Fourier transform infrared (FTIR) microscopy-based imaging protocol was developed for the simultaneous cellular quota quantification of lipids, carbohydrates, and proteins of the diatom
Identifiants
pubmed: 34858459
doi: 10.3389/fpls.2021.756421
pmc: PMC8631545
doi:
Types de publication
Journal Article
Langues
eng
Pagination
756421Informations de copyright
Copyright © 2021 Scarsini, Thurotte, Veidl, Amiard, Niepceron, Badawi, Lagarde, Schoefs and Marchand.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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