Mechanical characterization of rose bengal and green light crosslinked collagen scaffolds for regenerative medicine.
cell–collagen interactions
collagen laminates
collagen type I
micro tensile testing
rose bengal and green light crosslinking
thickness analysis
Journal
Regenerative biomaterials
ISSN: 2056-3418
Titre abrégé: Regen Biomater
Pays: England
ID NLM: 101652150
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
19
07
2021
revised:
30
09
2021
accepted:
22
10
2021
entrez:
3
12
2021
pubmed:
4
12
2021
medline:
4
12
2021
Statut:
epublish
Résumé
Collagen is one of the most important biomaterials for tissue engineering approaches. Despite its excellent biocompatibility, it shows the non-negligible disadvantage of poor mechanical stability. Photochemical crosslinking with rose bengal and green light (RGX) is an appropriate method to improve this property. The development of collagen laminates is helpful for further adjustment of the mechanical properties as well as the controlled release of incorporated substances. In this study, we investigate the impact of crosslinking and layering of two different collagen scaffolds on the swelling behavior and mechanical behavior in micro tensile tests to obtain information on its wearing comfort (stiffness, strength and ductility). The mechanical stability of the collagen material after degradation due to cell contact is examined using thickness measurements. There is no linear increase or decrease due to layering homologous laminates. Unexpectedly, a decrease in elongation at break, Young's modulus and ultimate tensile strength are measured when the untreated monolayer is compared to the crosslinked one. Furthermore, we can detect a connection between stability and cell proliferation. The results show that with variation in number and type of layers, collagen scaffolds with tailored mechanical properties can be produced. Such a multi-layered structure enables the release of biomolecules into inner or outer layers for biomedical applications.
Identifiants
pubmed: 34858633
doi: 10.1093/rb/rbab059
pii: rbab059
pmc: PMC8633790
doi:
Types de publication
Journal Article
Langues
eng
Pagination
rbab059Informations de copyright
© The Author(s) 2021. Published by Oxford University Press.
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