Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma.

DLBCL mouse models Lymphoma microenvironment cancer immunotherapy oncogenic STAT3 signaling personalized treatment

Journal

Oncoimmunology
ISSN: 2162-402X
Titre abrégé: Oncoimmunology
Pays: United States
ID NLM: 101570526

Informations de publication

Date de publication:
2021
Historique:
entrez: 3 12 2021
pubmed: 4 12 2021
medline: 4 1 2022
Statut: epublish

Résumé

Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy arising from germinal center or post-germinal center B-cells that retain many of the properties of normal B-cells. Here we show that a subset of DLBCL express the cytokine IL-10 and its receptor. The genetic ablation of IL-10 receptor signaling abrogates the autocrine STAT3 phosphorylation triggered by tumor cell-intrinsic IL-10 expression and impairs growth of DLBCL cell lines in subcutaneous and orthotopic xenotransplantation models. Furthermore, we demonstrate using an immunocompetent Myc-driven model of DLBCL that neutralization of IL-10 signaling reduces tumor growth, which can be attributed to reduced Treg infiltration, stronger intratumoral effector T-cell responses, and restored tumor-specific MHCII expression. The effects of IL-10R neutralization were phenocopied by the genetic ablation of IL-10 signaling in the Treg compartment and could be reversed by MHCII blockade. The BTK inhibitor ibrutinib effectively blocked tumor cell-intrinsic IL-10 expression and tumor growth in this Myc-driven model. Tumors from patients with high IL-10RA expression are infiltrated by higher numbers of Tregs than IL-10RA

Identifiants

pubmed: 34858727
doi: 10.1080/2162402X.2021.2003533
pii: 2003533
pmc: PMC8632300
doi:

Substances chimiques

IL10 protein, human 0
Interleukin-10 130068-27-8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2003533

Informations de copyright

© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

Déclaration de conflit d'intérêts

No potential conflict of interest was reported by the author(s).

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Auteurs

Kristin Stirm (K)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.

Peter Leary (P)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.

Katrin Bertram (K)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.

Nicolás Gonzalo Núñez (NG)

Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.

Daria Wüst (D)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.

Christophe Boudesco (C)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.

Els Verhoeyen (E)

CIRI, Université de Lyon; Inserm U1111; Ens de Lyon, France.
C3M, Université Côte d'Azur, INSERM, Nice, France.

Thorsten Zenz (T)

Department of Medical Oncology and Hematology, University Hospital Zurich and University of Zurich, Zurich, Switzerland.
Comprehensive Cancer Center Zurich, Zurich, Switzerland.

Burkhard Becher (B)

Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
Comprehensive Cancer Center Zurich, Zurich, Switzerland.

Thomas Menter (T)

Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Switzerland.

Alexandar Tzankov (A)

Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Switzerland.

Anne Müller (A)

Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.
Comprehensive Cancer Center Zurich, Zurich, Switzerland.

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