Direct somatic embryogenesis and flow cytometric assessment of ploidy stability in regenerants of Caladium × hortulanum 'Fancy'.
Direct somatic embryogenesis
Flow cytometry
Ploidy analysis
Somaclonal variations
Journal
Journal of applied genetics
ISSN: 2190-3883
Titre abrégé: J Appl Genet
Pays: England
ID NLM: 9514582
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
23
05
2021
accepted:
30
08
2021
revised:
12
08
2021
pubmed:
4
12
2021
medline:
7
4
2022
entrez:
3
12
2021
Statut:
ppublish
Résumé
Caladium × hortulanum 'Fancy' is an important ornamental plant grown in pots and landscapes and known for its colorful leaves often used for interior decorations. In this work, we present a method of in vitro regeneration from three explants source through direct somatic embryogenesis (DSE) wherein the regenerated plants were screened for ploidy changes through flow cytometry analysis. Tuber, leaf and petiole explants were cultured on MS basal medium supplemented with 1-napthalene acetic acid (NAA), 6-benzyl amino purine (BAP) and N-phenyl-N'-1, 2,3-thiadiazol-5-ylurea (TDZ) concentrations. Tuber explants induced highest direct somatic embryos on NAA (1 mg L
Identifiants
pubmed: 34859368
doi: 10.1007/s13353-021-00663-y
pii: 10.1007/s13353-021-00663-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
199-211Informations de copyright
© 2021. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences.
Références
Ahmed E, Hayashi T, Zhu Y, Hosokawa M, Yazawa S (2002) Lower incidence of variants in caladium bicolor ait. plants propagated by culture of explants from younger tissue. Sci Horticult 96(1-4):187–194
doi: 10.1016/S0304-4238(02)00092-4
Ahmed E, Hayashi T, Yazawa S (2004) Auxins increase the occurrence of leaf-colour variants in caladium regenerated from leaf explants. Sci Horticult 100(1-4):153–159
doi: 10.1016/j.scienta.2003.08.012
GBd A, Dibax R, RAd O, Bespalhok Filho J C, Daros E (2014) Plant regeneration and histological study of the somatic embryogenesis of sugarcane (saccharum spp.) cultivars rb855156 and rb72454. Acta Sci Agron 36(1):63–72
doi: 10.4025/actasciagron.v36i1.16342
Ali A, Munawar A, Naz S (2007) An in vitro study on micropropagation of caladium bicolor. Int J Agric Biol 9(5):731–735
Ali M, Mujib A, Tonk D, Zafar N (2017) Plant regeneration through somatic embryogenesis and genome size analysis of coriandrum sativum l. Protoplasma 254(1):343–352
doi: 10.1007/s00709-016-0954-2
Alizadeh M, Krishna H, Eftekhari M, Modareskia M, Modareskia M (2015) Assessment of clonal fidelity in micropropagated horticultural plants. J Chem Pharm Res 7(12):977–990
Bairu M W, Aremu A O, Van Staden J (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63(2):147–173
doi: 10.1007/s10725-010-9554-x
Bennett M, Leitch I (2012) .
Bhattacharyya P, Kumaria S, Job N, Tandon P (2016) En-masse production of elite clones of dendrobium crepidatum: a threatened, medicinal orchid used in traditional chinese medicine (tcm). J Appl Res Med Aromatic Plants 3(4):168–176
doi: 10.1016/j.jarmap.2016.04.001
Cai X, Cao Z, Xu S, Deng Z (2015) Induction, regeneration and characterization of tetraploids and variants in ‘tapestry’caladium. Plant Cell Tissue Organ Cult (PCTOC) 120(2):689–700
doi: 10.1007/s11240-014-0636-8
Cao Z, Deng Z, Mclaughlin M (2014) Interspecific genome size and chromosome number variation shed new light on species classification and evolution in caladium. J Am Soc Hortic Sci 139(4):449–459
doi: 10.21273/JASHS.139.4.449
Cao Z, Sui S, Cai X, Yang Q, Deng Z (2016) Somaclonal variation in ’red flash’caladium: morphological, cytological and molecular characterization. Plant Cell Tissue Organ Cult (PCTOC) 126(2):269–279
doi: 10.1007/s11240-016-0996-3
Chen J, Wei X (2018) Thidiazuron in micropropagation of aroid plants. In: Thidiazuron: From Urea Derivative to Plant Growth Regulator. Springer, pp 95–113
Deng Z (2012) Caladium genetics and breeding: recent advances. Floric Ornam Biotechnol 6:53–61
Dolezel J (2003) Nuclear dna content and genome size of trout and human. Cytometry Part A 51:127–128
doi: 10.1002/cyto.a.10013
Doležel J, Bartoš J (2005) Plant dna flow cytometry and estimation of nuclear genome size. Ann Botany 95(1):99–110
doi: 10.1093/aob/mci005
Doležel J, Greilhuber J, Suda J (2007) Estimation of nuclear dna content in plants using flow cytometry. Nat Protocols 2(9): 2233
doi: 10.1038/nprot.2007.310
Gholami A A, Alavi S V, Majd A, Fallahian F (2013) Plant regeneration through direct and indirect somatic embryogenesis from immature seeds of citrus. Eur J Exp Bio 3:307–10
Greilhuber J, Doležel J, Lysak MA, Bennett MD (2005) The origin, evolution and proposed stabilization of the terms ’genome size’and ’c-value’to describe nuclear dna contents. Ann Botany 95(1):255–260
doi: 10.1093/aob/mci019
Gulzar B, Mujib A, Malik M Q, Sayeed R, Mamgain J, Ejaz B (2020) Genes, proteins and other networks regulating somatic embryogenesis in plants. J Gen Eng Biotechnol 18(1):1–15
doi: 10.1186/s43141-019-0015-2
Henny R, Norman D, Chen J (2004) Progress in ornamental aroid breeding research. Ann Mo Bot Gard:464–472
Johansen D (1940) Botanical microtechnique. McCraw-Hill, New York
Kulus D (2016) Somatic embryogenesis in ornamentals and its applications. Springer, India. chap Application of cryogenic technologies and somatic embryogenesis in the storage and protection of valuable genetic resources of ornamental plants
Kumar M S, Nandi S C (2015) High frequency plant regeneration with histological analysis of organogenic callus from internode explants of asteracantha longifolia nees. J Gen Eng Biotechnol 13(1):31–37
doi: 10.1016/j.jgeb.2014.12.002
Liu W, Liang Z, Shan C, Marsolais F, Tian L (2013) Genetic transformation and full recovery of alfalfa plants via secondary somatic embryogenesis. Vitro Cell Dev Biol-Plant 49(1):17–23
doi: 10.1007/s11627-012-9463-y
Loureiro J, Trávníc~ek P, Rauchová J, Urfus T, Vit P, c~tech M, Castro S, Suda J et al (2010) The use of flow cytometry in the biosystematics, ecology and population biology of homoploid plants. Preslia 82(1):3–21
Malik M Q, Mujib A, Gulzar B, Zafar N, Syeed R, Mamgain J, Ejaz B (2020) Genome size analysis of field grown and somatic embryo regenerated plants in allium sativum l. J Appl Genet 61 (1):25–35
doi: 10.1007/s13353-019-00536-5
Maqsood M, MUJIB A, KHUSRAU M (2015) Preparation and low temperature short-term storage for synthetic seeds of caladium bicolor. Notulae Sci Biol 7(1):90–95
doi: 10.15835/nsb719405
Méndez-Hernández HA, Ledezma-Rodríguez M, Avilez-Montalvo RN, Juárez-Gómez YL, Skeete A, Avilez-Montalvo J, De-la Peña C, Loyola-Vargas VM (2019) Signaling overview of plant somatic embryogenesis. Front Plant Sci 10:77
Meziane M, Frasheri D, Carra A, Boudjeniba M, D’Onghia A M, Mercati F, Djelouah K, Carimi F (2017) Attempts to eradicate graft-transmissible infections through somatic embryogenesis in citrus ssp. and analysis of genetic stability of regenerated plants. Eur J Plant Pathol 148(1):85–95
doi: 10.1007/s10658-016-1072-x
Mujib A, Banerjee S, Fatima S, Ghosh P D (2008) Regenerated plant populations from rhizome-calli showed morphological and chromosomal changes in caladium bicolor (ait.) vent. cv.’ Bleed Heart Prop Ornament Plants 8(3):138–143
Mujib A, Pipal T, Ali M, Tonk D, Zafar N, Gulzar B (2017) In vitro propagation of althaea officinalis: the role of plant growth regulators in morphogenesis. BioTechnol J Biotechnol Comput Biol Bionanotechnol 98(3)
Nath S, Mallick S K, Jha S (2014) An improved method of genome size estimation by flow cytometry in five mucilaginous species of hyacinthaceae. Cytometry Part A 85(10):833–840
doi: 10.1002/cyto.a.22489
Raji M R, Lotfi M, Tohidfar M, Zahedi B, Carra A, Abbate L, Carimi F (2018) Somatic embryogenesis of muskmelon (cucumis melo l.) and genetic stability assessment of regenerants using flow cytometry and issr markers. Protoplasma 255(3):873–883
doi: 10.1007/s00709-017-1194-9
Ramakrishna D, Shasthree T (2016) High efficient somatic embryogenesis development from leaf cultures of citrullus colocynthis (l.) schrad for generating true type clones. Physiol Mol Biol Plants 22(2):279–285
doi: 10.1007/s12298-016-0357-z
Rivas E, Duarte L, Alexandre M, Galleti S, Harakava R, Fernandes F (2005) Caladium virus x, a new potexvirus from caladium bicolor (araceae). J Plant Pathol:109–114
Seydi S, Negahdar N, Taghizadeh Andevari R, Ansari M H, Kaviani B (2016) Effect of bap and naa on micropropagation of caladium bicolor (aiton) vent., an ornamental plant. J Ornamental Plants 6(1):59–66
Sherif N A, Benjamin J F, Kumar T S, Rao M (2018) Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of anoectochilus elatus lindl., an endangered terrestrial jewel orchid. Plant Cell Tissue Organ Cult (PCTOC) 132(2):303–316
doi: 10.1007/s11240-017-1330-4
Syeed R, Mujib A, Malik M Q, Mamgain J, Ejaz B, Gulzar B, Zafar N (2021) Mass propagation through direct and indirect organogenesis in three species of genus zephyranthes and ploidy assessment of regenerants through flow cytometry. Mol Biol Rep 48(1):513–526
doi: 10.1007/s11033-020-06083-1
Thepsithar C, Thongpukdee A, Chiensil P (2009) Micropropagation of caladium bicolor (ait.) vent.’thep songsil’and incidence of somaclonal variants. In: XXIII International EUCARPIA symposium, section ornamentals, colourful breeding and genetics-part II 855, pp 273–280
Thongpukdee A, Thepsithar C, Chiensil P, et al. (2010) Somaclonal variation of caladium bicolor (ait.) vent.’jao ying’after in vitro culture propagation. Acta Horticult (855):281–288
Tosoc J P, Frediles V C, Canda C, Demayo C G (2016) Antiangiogenic, antitoxic and antioxidant properties of methanolic extracts of caladium bicolor (aiton) venten. Human Veterinary Med 8(1):10–16
Tran T, Bui T, Feng T (2014) The role of auxin and cytokinin on somatic embryogenesis from cell suspension cultures of the banana cultivar’cau man’. In: XXIX International horticultural congress on horticulture: Sustaining lives, livelihoods and landscapes (IHC2014): IX 1114, pp 219–226
Zafar N, Mujib A, Ali M, Tonk D, Gulzar B, Malik M, Sayeed R, Mamgain J (2019) Genome size analysis of field grown and tissue culture regenerated rauvolfia serpentina (l) by flow cytometry: Histology and scanning electron microscopic study for in vitro morphogenesis. Ind Crop Prod 128:545–555
doi: 10.1016/j.indcrop.2018.11.049
Zhao J, Cui J, Liu J, Liao F, Henny RJ, Chen J (2012) Direct somatic embryogenesis from leaf and petiole explants of spathiphyllum †s~upreme’ and analysis of regenerants using flow cytometry. Plant Cell Tissue Organ Cult (PCTOC) 110(2):239–249. https://doi.org/10.1007/s11240-012-0146-5
Zhong L, Liu E, Yang C, Jin S, Diao Y, Hu Z (2017) High embryogenic ability and regeneration from floral axis of amorphophallus konjac (araceae). Open Life Sci 12(1):34–41. https://doi.org/10.1515/biol-2017-0004 . https://www.degruyter.com/view/journals/biol/12/1/article-p34.xml