Disinfection efficacy and mechanism of olanexidine gluconate against norovirus.
Adenovirus
Antiseptic
Non-enveloped virus
Norovirus
Olanexidine
Virus-like particle
Journal
American journal of infection control
ISSN: 1527-3296
Titre abrégé: Am J Infect Control
Pays: United States
ID NLM: 8004854
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
23
08
2021
revised:
18
11
2021
accepted:
19
11
2021
pubmed:
6
12
2021
medline:
24
6
2022
entrez:
5
12
2021
Statut:
ppublish
Résumé
The purpose of this study was to evaluate the virucidal activity of a new olanexidine-containing formulation for hand hygiene (olanexidine gluconate hand rub; OLG-HR) against non-enveloped viruses and to understand its mechanism of action. The virucidal activities of OLG-HR against two strains of caliciviruses and three adenovirus serotypes were evaluated through suspension tests. Also, virus-like particles were used to predict the effect of olanexidine gluconate on virus particle structure. The results of suspension tests under conditions with and without interfering substances (1.5% BSA) indicated that OLG-HR had a broad-spectrum effect against non-enveloped viruses, and the virucidal effect was unaffected by organic contaminants. Furthermore, olanexidine inhibited the binding ability of virus-like particles to the binding receptor of human norovirus and increased the aggregation of virus-like particles in a dose-dependent manner. Transmission electron microscopy showed that the morphology of the virus-like particles was affected by exposure to olanexidine, indicating that the protein-denaturing effect of olanexidine gluconate caused the loss of receptor-binding capability of the viral capsid protein. This study suggests that olanexidine gluconate is a potential biological and environmental disinfectant against norovirus and adenovirus.
Sections du résumé
BACKGROUND
The purpose of this study was to evaluate the virucidal activity of a new olanexidine-containing formulation for hand hygiene (olanexidine gluconate hand rub; OLG-HR) against non-enveloped viruses and to understand its mechanism of action.
METHODS
The virucidal activities of OLG-HR against two strains of caliciviruses and three adenovirus serotypes were evaluated through suspension tests. Also, virus-like particles were used to predict the effect of olanexidine gluconate on virus particle structure.
RESULTS
The results of suspension tests under conditions with and without interfering substances (1.5% BSA) indicated that OLG-HR had a broad-spectrum effect against non-enveloped viruses, and the virucidal effect was unaffected by organic contaminants. Furthermore, olanexidine inhibited the binding ability of virus-like particles to the binding receptor of human norovirus and increased the aggregation of virus-like particles in a dose-dependent manner. Transmission electron microscopy showed that the morphology of the virus-like particles was affected by exposure to olanexidine, indicating that the protein-denaturing effect of olanexidine gluconate caused the loss of receptor-binding capability of the viral capsid protein.
CONCLUSIONS
This study suggests that olanexidine gluconate is a potential biological and environmental disinfectant against norovirus and adenovirus.
Identifiants
pubmed: 34864086
pii: S0196-6553(21)00787-2
doi: 10.1016/j.ajic.2021.11.020
pii:
doi:
Substances chimiques
Anti-Infective Agents, Local
0
Biguanides
0
Disinfectants
0
Glucuronates
0
olanexidine gluconate
I69T4JE922
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
764-771Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.