Stabilization of Ribosomal RNA of the Small Subunit by Spermidine in
RNA stability
Staphylococcus aureus
ribosomal RNA
ribosome 70 S
translation
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
09
07
2021
accepted:
07
10
2021
entrez:
6
12
2021
pubmed:
7
12
2021
medline:
7
12
2021
Statut:
epublish
Résumé
Cryo-electron microscopy is now used as a method of choice in structural biology for studying protein synthesis, a process mediated by the ribosome machinery. In order to achieve high-resolution structures using this approach, one needs to obtain homogeneous and stable samples, which requires optimization of ribosome purification in a species-dependent manner. This is especially critical for the bacterial small ribosomal subunit that tends to be unstable in the absence of ligands. Here, we report a protocol for purification of stable 30 S from the Gram-positive bacterium
Identifiants
pubmed: 34869582
doi: 10.3389/fmolb.2021.738752
pii: 738752
pmc: PMC8637172
doi:
Types de publication
Journal Article
Langues
eng
Pagination
738752Informations de copyright
Copyright © 2021 Belinite, Khusainov, Soufari, Marzi, Romby, Yusupov and Hashem.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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