Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with abnormal accumulation of specific lipids.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 12 2021
06 12 2021
Historique:
received:
21
06
2021
accepted:
16
11
2021
entrez:
7
12
2021
pubmed:
8
12
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Ligamentum flavum hypertrophy (HLF) is the most important component of lumbar spinal canal stenosis (LSCS). Analysis of hypertrophied ligamentum flavum (HLF) samples from patients with LSCS can be an important que. The current study analyzed the surgical samples of HLF samples in patients with LCSC using quantitative and qualitative high performance-liquid chromatography and mass spectrometry. We collected ligamentum flavum (LF) tissue from twelve patients with LSCS and from four patients with lumbar disk herniation (LDH). We defined LF from LSCS patients as HLF and that from LDH patients as non-hypertrophied ligamentum flavum (NHLF). Total lipids were extracted from the LF samples and evaluated for quantity and quality using liquid chromatography and mass spectrometry. The total lipid amount of the HLF group was 3.6 times higher than that of the NHLF group. Phosphatidylcholines (PCs), ceramides (Cers), O-acyl-ω-hydroxy fatty acids (OAHFAs), and triglycerides (TGs) in the HLF group were more than 32 times higher than those of the NHLF group. PC(26:0)+H+, PC(25:0)+H+, and PC(23:0)+H+ increased in all patients in the HLF group compared to the NHLF group. The thickness of the LF correlated significantly with PC(26:0)+H+ in HLF. We identified the enriched specific PCs, Cers, OAHFAs, and TGs in HLF.
Identifiants
pubmed: 34873238
doi: 10.1038/s41598-021-02818-7
pii: 10.1038/s41598-021-02818-7
pmc: PMC8648848
doi:
Substances chimiques
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
23515Informations de copyright
© 2021. The Author(s).
Références
Int J Mol Sci. 2017 Mar 27;18(4):
pubmed: 28346372
J Spinal Disord. 1995 Apr;8(2):126-30
pubmed: 7606119
Clin Chim Acta. 2014 Apr 20;431:221-6
pubmed: 24530297
Eur Spine J. 2009 May;18(5):679-86
pubmed: 19277726
Rapid Commun Mass Spectrom. 2004;18(20):2395-400
pubmed: 15386630
Proc Jpn Acad Ser B Phys Biol Sci. 2017;93(9):677-702
pubmed: 29129849
Biosci Rep. 2018 Mar 29;38(2):
pubmed: 29436483
Spine (Phila Pa 1976). 1992 Nov;17(11):1353-60
pubmed: 1462211
Clin Orthop Surg. 2009 Jun;1(2):81-9
pubmed: 19885059
Rapid Commun Mass Spectrom. 2014 Jul 15;28(13):1453-8
pubmed: 24861594
Elife. 2019 Aug 16;8:
pubmed: 31418690
Cell Physiol Biochem. 2018;45(4):1472-1486
pubmed: 29466791
Int J Mol Sci. 2014 Feb 20;15(2):2991-3002
pubmed: 24562328
Clin Med Insights Endocrinol Diabetes. 2016 May 05;9:1-5
pubmed: 27168730
Spine (Phila Pa 1976). 2013 Apr 20;38(9):E540-5
pubmed: 23380819
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
PLoS One. 2018 Aug 1;13(8):e0200872
pubmed: 30067795
J Orthop Res. 2016 Jul;34(7):1241-7
pubmed: 26679090
Clin Anat. 2010 Jan;23(1):79-83
pubmed: 19941359
Spine (Phila Pa 1976). 2001 Nov 1;26(21):E492-5
pubmed: 11679833
Ocul Surf. 2020 Oct;18(4):545-553
pubmed: 32562857
Spine (Phila Pa 1976). 2015 Sep 15;40(18):1451-6
pubmed: 26165225
J Orthop Sci. 2017 Mar;22(2):190-196
pubmed: 27889106
J Orthop Sci. 2019 Jan;24(1):19-23
pubmed: 30243520
Spine (Phila Pa 1976). 2018 Apr 15;43(8):E468-E473
pubmed: 28858184
Spine (Phila Pa 1976). 2005 Dec 1;30(23):2649-56
pubmed: 16319751