Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.
Embryonic stem cells
Epiblast
Mammalian embryo
Nuclear transfer
Pluripotency
Self-renewal
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
15
06
2021
accepted:
26
10
2021
entrez:
7
12
2021
pubmed:
8
12
2021
medline:
3
2
2022
Statut:
ppublish
Résumé
Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the embryo uncertain. Here, we avoided using feeder cells or serum factors to provide a defined culture microenvironment. We show that the combination of activin A, fibroblast growth factor and the Wnt inhibitor XAV939 (AFX) supports establishment and continuous expansion of pluripotent stem cell lines from porcine, ovine and bovine embryos. Germ layer differentiation was evident in teratomas and readily induced in vitro. Global transcriptome analyses highlighted commonality in transcription factor expression across the three species, while global comparison with porcine embryo stages showed proximity to bilaminar disc epiblast. Clonal genetic manipulation and gene targeting were exemplified in porcine stem cells. We further demonstrated that genetically modified AFX stem cells gave rise to cloned porcine foetuses by nuclear transfer. In summary, for major livestock mammals, pluripotent stem cells related to the formative embryonic disc are reliably established using a common and defined signalling environment. This article has an associated 'The people behind the papers' interview.
Identifiants
pubmed: 34874452
pii: 273644
doi: 10.1242/dev.199901
pmc: PMC8714072
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100526/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P009867/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S020845/2
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S000178/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S020845/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100526
Pays : United Kingdom
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests M.K., R.A. and A.S. may benefit from commercial licensing of EDSCs by the University of Nottingham and the University of Cambridge.
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