An efficient, non-invasive approach for
DNA damage response
cellular senescence
hair follicle cells
irradiation
non-invasive sampling
Journal
Aging
ISSN: 1945-4589
Titre abrégé: Aging (Albany NY)
Pays: United States
ID NLM: 101508617
Informations de publication
Date de publication:
06 12 2021
06 12 2021
Historique:
received:
15
07
2021
accepted:
22
11
2021
pubmed:
8
12
2021
medline:
19
2
2022
entrez:
7
12
2021
Statut:
ppublish
Résumé
In accordance with the 3 Rs principle (to replace, reduce and refine) animal models in biomedical research, we have developed and applied a new approach for sampling and analyzing hair follicles in various experimental settings. This involves use of a convenient device for non-invasive collection of hair follicles and processing methods that provide sufficient amounts of biological material to replace stressful and painful biopsies. Moreover, the main components of hair follicles are live cells of epithelial origin, which are highly relevant for most types of malignant tumors, so they provide opportunities for studying aging-related pathologies including cancer. Here, we report the successful use of the method to obtain mouse hair follicular cells for genotyping, quantitative PCR, and quantitative immunofluorescence. We present proof of concept data demonstrating its utility for routine genotyping and monitoring changes in quality and expression levels of selected proteins in mice after gamma irradiation and during natural or experimentally induced aging. We also performed pilot translation of animal experiments to human hair follicles irradiated
Identifiants
pubmed: 34874896
pii: 203744
doi: 10.18632/aging.203744
pmc: PMC8714131
doi:
Banques de données
figshare
['10.6084/m9.figshare.14822643.v1']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
25004-25024Références
Radiat Res. 2015 Mar;183(3):325-37
pubmed: 25738893
Cell Cycle. 2011 Apr 1;10(7):1152-61
pubmed: 21389785
Int J Mol Sci. 2018 Sep 27;19(10):
pubmed: 30261683
Mech Ageing Dev. 2013 Jan-Feb;134(1-2):35-42
pubmed: 23262094
Sci Rep. 2017 Jul 28;7(1):6824
pubmed: 28755004
J Invest Dermatol. 2013 Sep;133(9):2143-51
pubmed: 23549419
Aging Cell. 2017 Feb;16(1):192-197
pubmed: 28165661
Science. 1997 Aug 8;277(5327):831-4
pubmed: 9242615
Nat Rev Cancer. 2010 Jan;10(1):51-7
pubmed: 20029423
Radiat Res. 2010 Oct;174(4):415-23
pubmed: 20726705
Nat Cell Biol. 2009 Aug;11(8):973-9
pubmed: 19597488
Aging (Albany NY). 2016 Jul;8(7):1294-315
pubmed: 27391570
Int J Radiat Oncol Biol Phys. 2008 Jul 15;71(4):1236-1244
pubmed: 18572087
Oncogene. 1999 Jul 8;18(27):3930-5
pubmed: 10435615
J Vet Sci. 2012 Dec;13(4):331-8
pubmed: 23271173
Aging (Albany NY). 2016 Apr;8(4):664-84
pubmed: 27013617
N Engl J Med. 1999 Aug 12;341(7):491-7
pubmed: 10441606
Cancer Cell. 2011 Jan 18;19(1):114-24
pubmed: 21215705
Eur J Biochem. 2001 May;268(10):2784-91
pubmed: 11358493
In Vivo. 2000 Jan-Feb;14(1):183-8
pubmed: 10757076
Lab Anim. 2004 Oct;38(4):413-7
pubmed: 15479556
Cell Death Dis. 2020 Oct 14;11(10):854
pubmed: 33056980
Aging Cell. 2006 Oct;5(5):379-89
pubmed: 16911562
Oncol Rep. 2014 May;31(5):2229-35
pubmed: 24626611
Cell Death Dis. 2019 Feb 27;10(3):199
pubmed: 30814491
Cell Cycle. 2012 Oct 1;11(19):3599-610
pubmed: 22935696
Proc Natl Acad Sci U S A. 2019 Feb 12;116(7):2603-2611
pubmed: 30683717
PLoS One. 2010 Nov 23;5(11):e15544
pubmed: 21124906
Radiat Res. 2010 Jan;173(1):1-9
pubmed: 20041754
Cancer Lett. 2011 Mar 1;302(1):20-8
pubmed: 21236564
Front Pharmacol. 2018 May 22;9:522
pubmed: 29872395
Lab Anim. 1999 Oct;33(4):385-9
pubmed: 10778788
Toxicol Pathol. 2007 Dec;35(7):952-7
pubmed: 18098041
Radiat Res. 2012 Jan;177(1):69-83
pubmed: 22029842
Nat Med. 2000 Aug;6(8):849-51
pubmed: 10932210
Cell. 2000 Aug 18;102(4):407-10
pubmed: 10966103
Nat Med. 2013 Jul;19(7):924-9
pubmed: 23749232
J Invest Dermatol. 1986 Dec;87(6):768-70
pubmed: 3782859
Nat Cell Biol. 2019 Jan;21(1):94-101
pubmed: 30602768
EMBO J. 2004 Jul 7;23(13):2554-63
pubmed: 15192702
Radiat Res. 2009 Sep;172(3):306-13
pubmed: 19708779
J Circadian Rhythms. 2012 Oct 05;10(1):7
pubmed: 23039139