Rapid and robust optogenetic control of gene expression in Drosophila.
Drosophila
Gal4/UAS
gene expression
optogenetics
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
20 12 2021
20 12 2021
Historique:
received:
27
05
2021
revised:
13
10
2021
accepted:
15
11
2021
pubmed:
9
12
2021
medline:
13
1
2022
entrez:
8
12
2021
Statut:
ppublish
Résumé
Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions.
Identifiants
pubmed: 34879263
pii: S1534-5807(21)00940-0
doi: 10.1016/j.devcel.2021.11.016
pmc: PMC8693864
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3393-3404.e7Subventions
Organisme : Wellcome Trust
ID : 206341/Z/17/Z
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001204
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001204
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001204
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001204
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests Yohanns Bellaiche is a Developmental Cell advisory board member.
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