The effect of urine storage temperature and boric acid preservation on quantitative bacterial culture for diagnosing canine urinary tract infection.
Dog
Refrigeration
Significant bacteriuria
Urine cultures
Urine preservation
Urine transportation
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
08 Dec 2021
08 Dec 2021
Historique:
received:
22
06
2021
accepted:
18
11
2021
entrez:
9
12
2021
pubmed:
10
12
2021
medline:
4
3
2022
Statut:
epublish
Résumé
Quantitative bacterial culture (QBC) is the gold standard for diagnosing canine urinary tract infection. Current guidelines recommend QBC within 24 h of urine collection and that unpreserved urine is refrigerated until culture. However, temperature-controlled transport is rarely feasible, indicating a need for alternative storage during transport of urine from primary veterinary practices to the microbiology laboratory. The objective was to investigate the effect of storage temperature and boric acid sponge-preservation on quantitative bacterial culture of canine urine. Significant bacteriuria was detected in 72 out of 179 samples (40%) collected from 141 dogs. Overall accuracy was 94-98% for both storage conditions and time points. Non-inferiority (15% margin) to reference quantitative bacterial culture was evident for sensitivity, specificity and predictive values for both storage methods and time points, except for the negative predictive value for 48 h boric acid preservation (NPV: 89, 95% CI [79;95]). There was no significant difference between the sensitivity and specificity for either of the time-points (p-value = 0.07-1). Boric acid sponge-preservation using Uriswab™ is a useful alternative to refrigeration of urine samples during transport. Reliable quantitative bacterial culture results can be obtained from canine urine up to 48 h after collection if urine is refrigerated, and for at least 24 h if urine is stored using a boric acid-containing urine transport system.
Sections du résumé
BACKGROUND
BACKGROUND
Quantitative bacterial culture (QBC) is the gold standard for diagnosing canine urinary tract infection. Current guidelines recommend QBC within 24 h of urine collection and that unpreserved urine is refrigerated until culture. However, temperature-controlled transport is rarely feasible, indicating a need for alternative storage during transport of urine from primary veterinary practices to the microbiology laboratory. The objective was to investigate the effect of storage temperature and boric acid sponge-preservation on quantitative bacterial culture of canine urine.
RESULTS
RESULTS
Significant bacteriuria was detected in 72 out of 179 samples (40%) collected from 141 dogs. Overall accuracy was 94-98% for both storage conditions and time points. Non-inferiority (15% margin) to reference quantitative bacterial culture was evident for sensitivity, specificity and predictive values for both storage methods and time points, except for the negative predictive value for 48 h boric acid preservation (NPV: 89, 95% CI [79;95]). There was no significant difference between the sensitivity and specificity for either of the time-points (p-value = 0.07-1).
CONCLUSIONS
CONCLUSIONS
Boric acid sponge-preservation using Uriswab™ is a useful alternative to refrigeration of urine samples during transport. Reliable quantitative bacterial culture results can be obtained from canine urine up to 48 h after collection if urine is refrigerated, and for at least 24 h if urine is stored using a boric acid-containing urine transport system.
Identifiants
pubmed: 34879836
doi: 10.1186/s12917-021-03083-6
pii: 10.1186/s12917-021-03083-6
pmc: PMC8653607
doi:
Substances chimiques
Boric Acids
0
boric acid
R57ZHV85D4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
379Informations de copyright
© 2021. The Author(s).
Références
J Infect. 1989 Jan;18(1):51-8
pubmed: 2915130
Clin Microbiol Rev. 2016 Jan;29(1):105-47
pubmed: 26598386
Vet J. 2016 Oct;216:168-73
pubmed: 27687946
Diagn Microbiol Infect Dis. 2018 Nov;92(3):179-182
pubmed: 29941365
J Am Vet Med Assoc. 1987 May 15;190(10):1289-91
pubmed: 3294768
J Am Vet Med Assoc. 1981 May 15;178(10):1077-81
pubmed: 7024224
Scand J Clin Lab Invest. 2002;62(5):325-35
pubmed: 12387577
Zentralbl Veterinarmed B. 1992 Nov;39(9):662-7
pubmed: 1492522
J Clin Microbiol. 1979 Jul;10(1):42-5
pubmed: 500793
Vet J. 2019 May;247:8-25
pubmed: 30971357
J Am Vet Med Assoc. 2016 Jan 15;248(2):183-7
pubmed: 26720084
BMC Vet Res. 2014 Sep 24;10:217
pubmed: 25249356
Diagn Microbiol Infect Dis. 2016 Aug;85(4):401-4
pubmed: 27233427
J Small Anim Pract. 2011 Oct;52(10):510-4
pubmed: 21967098
Am J Clin Pathol. 2013 Sep;140(3):306-13
pubmed: 23955448