Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function.
Journal
NPJ biofilms and microbiomes
ISSN: 2055-5008
Titre abrégé: NPJ Biofilms Microbiomes
Pays: United States
ID NLM: 101666944
Informations de publication
Date de publication:
08 12 2021
08 12 2021
Historique:
received:
11
03
2021
accepted:
03
11
2021
entrez:
9
12
2021
pubmed:
10
12
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Bacterial biofilms represent a challenge to the healthcare system because of their resilience against antimicrobials and immune attack. Biofilms consist of bacterial aggregates embedded in an extracellular polymeric substance (EPS) composed of polysaccharides, nucleic acids and proteins. We hypothesised that carbohydrates could contribute to immune recognition of Pseudomonas aeruginosa biofilms by engaging C-type lectins. Here we show binding of Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN, CD209), mannose receptor (MR, CD206) and Dectin-2 to P. aeruginosa biofilms. We also demonstrate that DC-SIGN, unlike MR and Dectin-2, recognises planktonic P. aeruginosa cultures and this interaction depends on the presence of the common polysaccharide antigen. Within biofilms DC-SIGN, Dectin-2 and MR ligands appear as discrete clusters with dispersed DC-SIGN ligands also found among bacterial aggregates. DC-SIGN, MR and Dectin-2 bind to carbohydrates purified from P. aeruginosa biofilms, particularly the high molecular weight fraction (HMW; >132,000 Da), with K
Identifiants
pubmed: 34880222
doi: 10.1038/s41522-021-00257-w
pii: 10.1038/s41522-021-00257-w
pmc: PMC8655052
doi:
Substances chimiques
Carbohydrates
0
Lectins, C-Type
0
Mannose Receptor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
87Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM083016
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM119197
Pays : United States
Organisme : RCUK | Medical Research Council (MRC)
ID : MR/P001033/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/R012415/1
Informations de copyright
© 2021. The Author(s).
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