Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
08 12 2021
08 12 2021
Historique:
received:
19
01
2021
accepted:
18
11
2021
entrez:
9
12
2021
pubmed:
10
12
2021
medline:
25
12
2021
Statut:
epublish
Résumé
Cholesterol-rich microdomains are membrane compartments characterized by specific lipid and protein composition. These dynamic assemblies are involved in several biological processes, including infection by intracellular pathogens. This work provides a comprehensive analysis of the composition of human erythrocyte membrane microdomains. Based on their floating properties, we also categorized the microdomain-associated proteins into clusters. Interestingly, erythrocyte microdomains include the vast majority of the proteins known to be involved in invasion by the malaria parasite Plasmodium falciparum. We show here that the Ecto-ADP-ribosyltransferase 4 (ART4) and Aquaporin 1 (AQP1), found within one specific cluster, containing the essential host determinant CD55, are recruited to the site of parasite entry and then internalized to the newly formed parasitophorous vacuole membrane. By generating null erythroid cell lines, we showed that one of these proteins, ART4, plays a role in P. falciparum invasion. We also found that genetic variants in both ART4 and AQP1 are associated with susceptibility to the disease in a malaria-endemic population.
Identifiants
pubmed: 34880413
doi: 10.1038/s42003-021-02900-w
pii: 10.1038/s42003-021-02900-w
pmc: PMC8655059
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1375Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI140751
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL139337
Pays : United States
Informations de copyright
© 2021. The Author(s).
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