Tuft Cells Increase Following Ovine Intestinal Parasite Infections and Define Evolutionarily Conserved and Divergent Responses.
G protein-coupled receptor
RNAscope
Tuft cell
gastrointestinal tract
immunity
immunohistochemistry
parasitic nematode
single cell RNA sequencing
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
22
09
2021
accepted:
05
11
2021
entrez:
9
12
2021
pubmed:
10
12
2021
medline:
15
2
2022
Statut:
epublish
Résumé
Helminth parasite infections of humans and livestock are a global health and economic problem. Resistance of helminths to current drug treatment is an increasing problem and alternative control approaches, including vaccines, are needed. Effective vaccine design requires knowledge of host immune mechanisms and how these are stimulated. Mouse models of helminth infection indicate that tuft cells, an unusual type of epithelial cell, may 'sense' infection in the small intestine and trigger a type 2 immune response. Currently nothing is known of tuft cells in immunity in other host species and in other compartments of the gastrointestinal (GI) tract. Here we address this gap and use immunohistochemistry and single cell RNA-sequencing to detail the presence and gene expression profile of tuft cells in sheep following nematode infections. We identify and characterize tuft cells in the ovine abomasum (true stomach of ruminants) and show that they increase significantly in number following infection with the globally important nematodes
Identifiants
pubmed: 34880874
doi: 10.3389/fimmu.2021.781108
pmc: PMC8646091
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
781108Subventions
Organisme : Wellcome Trust
ID : 211814
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 219530
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104111
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104111/Z/14/ZR
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Hildersley, McNeilly, Gillan, Otto, Löser, Gerbe, Jay, Maizels, Devaney and Britton.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Exp Med. 2006 Apr 17;203(4):1105-16
pubmed: 16606668
Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8287-92
pubmed: 24843119
Neuron. 2006 Jan 5;49(1):41-53
pubmed: 16387638
Parasit Vectors. 2013 May 27;6:153
pubmed: 23711194
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Nature. 2016 Jan 14;529(7585):221-5
pubmed: 26675736
Immunity. 2017 Dec 19;47(6):1024-1036
pubmed: 29262347
Parasite Immunol. 2010 Aug;32(8):541-8
pubmed: 20626809
Sci Rep. 2020 Jun 1;10(1):8877
pubmed: 32483224
Sci Immunol. 2021 Mar 5;6(57):
pubmed: 33674322
BMC Genomics. 2018 Jun 19;19(1):477
pubmed: 29914354
Anat Anz. 1979;146(2):141-51
pubmed: 507383
Oncogene. 2014 Sep 18;33(38):4675-84
pubmed: 24662814
Ann N Y Acad Sci. 2019 Nov;1456(1):96-108
pubmed: 31215653
J Immunol. 2001 Aug 15;167(4):2234-9
pubmed: 11490010
Nature. 2016 Jan 14;529(7585):226-30
pubmed: 26762460
Annu Rev Immunol. 2019 Apr 26;37:47-72
pubmed: 30379593
Histochem Cell Biol. 2011 Aug;136(2):191-204
pubmed: 21688022
Int J Parasitol. 2020 Jul;50(8):533-553
pubmed: 32569640
Front Physiol. 2020 Feb 07;11:32
pubmed: 32116750
PLoS Pathog. 2016 Nov 1;12(11):e1005998
pubmed: 27802350
Science. 2014 Jun 6;344(6188):1168-1173
pubmed: 24904168
J Immunol. 2019 Mar 1;202(5):1321-1329
pubmed: 30782851
Acta Pathol Microbiol Scand Suppl. 1956;39(Suppl 111):72-3
pubmed: 13372265
Parasite Immunol. 2010 May;32(5):361-9
pubmed: 20500665
Front Physiol. 2013 Apr 02;4:58
pubmed: 23565094
Vet Parasitol. 2012 May 4;186(1-2):70-8
pubmed: 22154968
Sci Rep. 2019 Nov 25;9(1):17466
pubmed: 31767912
Immunity. 2018 Jul 17;49(1):33-41.e7
pubmed: 30021144
Nat Biotechnol. 2019 Dec;37(12):1482-1492
pubmed: 31796933
Science. 2016 Mar 18;351(6279):1329-33
pubmed: 26847546
Immunohorizons. 2020 Jan 24;4(1):23-32
pubmed: 31980480
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5552-5557
pubmed: 29735652
Vet Res. 2019 Nov 29;50(1):104
pubmed: 31783921
Parasitology. 2010 Jan;137(1):159-71
pubmed: 19712539
Cell. 2018 Jul 12;174(2):271-284.e14
pubmed: 29887373
Int J Parasitol. 1973 Sep;3(5):589-97
pubmed: 4795374
Sci Immunol. 2021 Mar 5;6(57):
pubmed: 33674321
Int J Parasitol. 1996 May;26(5):499-508
pubmed: 8818729
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Sci Rep. 2017 May 10;7(1):1652
pubmed: 28490731
J Comp Neurol. 2008 Aug 10;509(5):514-25
pubmed: 18537122
Immunity. 2020 Mar 17;52(3):528-541.e7
pubmed: 32160525
Nature. 2017 Nov 16;551(7680):333-339
pubmed: 29144463
Cell Mol Life Sci. 2012 Sep;69(17):2907-17
pubmed: 22527717
Curr Opin Immunol. 2021 Feb;68:98-106
pubmed: 33166855
Cell Tissue Res. 2010 Mar;339(3):493-504
pubmed: 20063013
Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5564-5569
pubmed: 30819885
Nat Commun. 2017 Jan 16;8:14049
pubmed: 28091601
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Physiol Genomics. 2009 Apr 10;37(2):67-78
pubmed: 19208773
Nature. 2018 Aug;560(7718):319-324
pubmed: 30069044
Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9478-83
pubmed: 21606356