Stability of Erythrocyte-Derived Nanovesicles Assessed by Light Scattering and Electron Microscopy.
cellular vesicles
cryo-electron microscopy of extracellular vesicles
extracellular vesicle reference material
light scattering
nanovesicles
scanning electron microscopy of extracellular vesicles
vesicle characterization
vesicle shape
vesicle stability
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
25 Nov 2021
25 Nov 2021
Historique:
received:
27
09
2021
revised:
12
11
2021
accepted:
22
11
2021
entrez:
10
12
2021
pubmed:
11
12
2021
medline:
18
12
2021
Statut:
epublish
Résumé
Extracellular vesicles (EVs) are gaining increasing amounts of attention due to their potential use in diagnostics and therapy, but the poor reproducibility of the studies that have been conducted on these structures hinders their breakthrough into routine practice. We believe that a better understanding of EVs stability and methods to control their integrity are the key to resolving this issue. In this work, erythrocyte EVs (hbEVs) were isolated by centrifugation from suspensions of human erythrocytes that had been aged in vitro. The isolate was characterised by scanning (SEM) and cryo-transmission electron microscopy (cryo-TEM), flow cytometry (FCM), dynamic/static light scattering (LS), protein electrophoresis, and UV-V spectrometry. The hbEVs were exposed to various conditions (pH (4-10), osmolarity (50-1000 mOsm/L), temperature (15-60 °C), and surfactant Triton X-100 (10-500 μM)). Their stability was evaluated by LS by considering the hydrodynamic radius (
Identifiants
pubmed: 34884574
pii: ijms222312772
doi: 10.3390/ijms222312772
pmc: PMC8657685
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : EU Commission
ID : Ves4us, 801338
Organisme : Slovenian Research Agency
ID : P2-0232, P3-0388, P1-0201, P1-0391, P2-0123, L3-2621
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