Fresh and Cryopreserved Human Umbilical-Cord-Derived Mesenchymal Stromal Cells Attenuate Injury and Enhance Resolution and Repair following Ventilation-Induced Lung Injury.

Animals Cell Line, Tumor Cells, Cultured Cryopreservation / methods Culture Media, Conditioned Humans Lung / drug effects Male Mesenchymal Stem Cell Transplantation / methods Mesenchymal Stem Cells / cytology Rats Rats, Sprague-Dawley Respiratory Distress Syndrome / metabolism Umbilical Cord / cytology Ventilator-Induced Lung Injury / metabolism
acute respiratory distress syndrome cryopreservation injury mesenchymal stem/stromal cells tissue source ventilation-induced lung injury

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
27 Nov 2021
Historique:
received: 22 10 2021
revised: 25 11 2021
accepted: 26 11 2021
entrez: 10 12 2021
pubmed: 11 12 2021
medline: 27 1 2022
Statut: epublish

Résumé

Ventilator-induced lung injury (VILI) frequently worsens acute respiratory distress syndrome (ARDS) severity. Human mesenchymal stem/stromal cells (MSCs) offer considerable therapeutic promise, but the key impediments of clinical translation stem from limitations due to cell source and availability, and concerns regarding the loss of efficacy following cryopreservation. These experiments compared the efficacy of umbilical-cord-derived MSCs (UC-MSCs), a readily available and homogenous tissue source, to the previously more widely utilised bone-marrow-derived MSCs (BM-MSCs). We assessed their capacity to limit inflammation, resolve injury and enhance repair in relevant lung mechanical stretch models, and the impact of cryopreservation on therapeutic efficacy. In Conditioned medium from BM-MSCs and UC-MSCs comparably decreased stretch-induced pulmonary epithelial inflammation and cell death. BM-MSCs and UC-MSCs comparably enhanced wound resolution. In animals subjected to VILI, both fresh BM-MSCs and UC-MSCs enhanced injury resolution and repair, while cryopreserved UC-MSCs comparably retained their efficacy. Cryopreserved UC-MSCs can reduce stretch-induced inflammation and cell death, enhance wound resolution, and enhance injury resolution and repair following VILI. Cryopreserved UC-MSCs represent a more abundant, cost-efficient, less variable and equally efficacious source of therapeutic MSC product.

Sections du résumé

BACKGROUND BACKGROUND
Ventilator-induced lung injury (VILI) frequently worsens acute respiratory distress syndrome (ARDS) severity. Human mesenchymal stem/stromal cells (MSCs) offer considerable therapeutic promise, but the key impediments of clinical translation stem from limitations due to cell source and availability, and concerns regarding the loss of efficacy following cryopreservation. These experiments compared the efficacy of umbilical-cord-derived MSCs (UC-MSCs), a readily available and homogenous tissue source, to the previously more widely utilised bone-marrow-derived MSCs (BM-MSCs). We assessed their capacity to limit inflammation, resolve injury and enhance repair in relevant lung mechanical stretch models, and the impact of cryopreservation on therapeutic efficacy.
METHODS METHODS
In
RESULTS RESULTS
Conditioned medium from BM-MSCs and UC-MSCs comparably decreased stretch-induced pulmonary epithelial inflammation and cell death. BM-MSCs and UC-MSCs comparably enhanced wound resolution. In animals subjected to VILI, both fresh BM-MSCs and UC-MSCs enhanced injury resolution and repair, while cryopreserved UC-MSCs comparably retained their efficacy.
CONCLUSIONS CONCLUSIONS
Cryopreserved UC-MSCs can reduce stretch-induced inflammation and cell death, enhance wound resolution, and enhance injury resolution and repair following VILI. Cryopreserved UC-MSCs represent a more abundant, cost-efficient, less variable and equally efficacious source of therapeutic MSC product.

Identifiants

DOI: 10.3390/ijms222312842 PMID: 34884645 PMC: PMC8657992
pubmed: 34884645
pii: ijms222312842
doi: 10.3390/ijms222312842
pmc: PMC8657992
pii:
doi:

Substances chimiques

Culture Media, Conditioned 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : European Research Council
ID : ERC-2007-StG 207777
Pays : International
Organisme : Science Foundation Ireland
ID : 16/FRL/3845
Pays : Ireland
Organisme : Health Research Board
ID : HRA-POR-2015-1099
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 14/TIDA/2291
Pays : Ireland

Références

Am J Respir Crit Care Med. 2004 Jan 1;169(1):46-56
pubmed: 12958048
Anesthesiology. 2013 Apr;118(4):924-32
pubmed: 23377221
Thorax. 2012 Jun;67(6):496-501
pubmed: 22106021
Cytotherapy. 2012 Feb;14(2):147-52
pubmed: 22029655
Am J Respir Crit Care Med. 2010 Oct 15;182(8):1047-57
pubmed: 20558630
Methods Mol Biol. 1998;79:179-83
pubmed: 9463833
Cell Biol Int. 2013 Oct 7;:
pubmed: 24123669
Nat Med. 2009 Jan;15(1):42-9
pubmed: 19098906
Am J Respir Crit Care Med. 2009 Dec 1;180(11):1122-30
pubmed: 19713447
Intensive Care Med. 2020 Dec;46(12):2265-2283
pubmed: 32654006
Stem Cells Dev. 2016 Apr 15;25(8):586-97
pubmed: 26914168
Cytotherapy. 2017 Jul;19(7):798-807
pubmed: 28462821
J Cell Biochem. 2012 Feb;113(2):658-68
pubmed: 21976004
Br J Haematol. 1999 Nov;107(2):275-81
pubmed: 10583212
Intensive Care Med Exp. 2020 Feb 5;8(1):8
pubmed: 32025852
Crit Care Med. 2017 Feb;45(2):e202-e212
pubmed: 27861182
Am J Physiol Lung Cell Mol Physiol. 2007 Jul;293(1):L131-41
pubmed: 17416739
Am J Respir Crit Care Med. 2009 Dec 1;180(11):1131-42
pubmed: 19713449
Front Immunol. 2015 Nov 03;6:560
pubmed: 26579133
Adv Physiol Educ. 2004 Dec;28(1-4):29-32
pubmed: 14973009
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8407-11
pubmed: 12815096
J Immunol. 2007 Aug 1;179(3):1855-63
pubmed: 17641052
Am J Respir Crit Care Med. 2013 Apr 1;187(7):751-60
pubmed: 23292883
Crit Care Med. 1996 Aug;24(8):1285-92
pubmed: 8706481
Behav Res Methods. 2007 May;39(2):175-91
pubmed: 17695343
Anesthesiology. 2015 Feb;122(2):363-73
pubmed: 25490744
Cytotherapy. 2013 Jan;15(1):2-8
pubmed: 23260081
Stem Cell Res Ther. 2019 Mar 18;10(1):99
pubmed: 30885254
Thorax. 2015 Jul;70(7):625-35
pubmed: 25986435
Intensive Care Med Exp. 2016 Dec;4(1):8
pubmed: 27001525
Stem Cell Res Ther. 2020 Mar 13;11(1):116
pubmed: 32169108
Transl Res. 2009 Nov;154(5):228-40
pubmed: 19840764
Int J Mol Sci. 2013 Sep 03;14(9):17986-8001
pubmed: 24005862

Auteurs

Shahd Horie (S)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.

Hector Gonzalez (H)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.

Jack Brady (J)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.

James Devaney (J)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.

Michael Scully (M)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Medicine, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.

Daniel O'Toole (D)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.

John G Laffey (JG)

Anaesthesia, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Regenerative Medicine Institute (REMEDI), CÚRAM Centre for Research in Medical Devices, National University of Ireland Galway, Biomedical Sciences Building, H91 TK33 Galway, Ireland.
Medicine, School of Medicine, Clinical Sciences Institute, National University of Ireland, H91 TK33 Galway, Ireland.
Department of Anaesthesia, Galway University Hospitals, SAOLTA University Health Group, H91 YR71 Galway, Ireland.
ORCID: 0000-0002-1246-9573

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Fresh and Cryopreserved Human...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Fresh and Cryopreserved Human...
questionsmedicales.fr › Cellules › Cellules cultivées › Fresh and Cryopreserved Human...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › Fresh and Cryopreserved Human...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Milieux de culture conditionnés › Fresh and Cryopreserved Human...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Fresh and Cryopreserved Human...
questionsmedicales.fr › Appareil respiratoire › Poumon › Fresh and Cryopreserved Human...
questionsmedicales.fr › Procédures de chirurgie opératoire › Transplantation › Transplantation cellulaire › Transplantation de cellules souches › Transplantation de cellules souches mésenchymateuses › Fresh and Cryopreserved Human...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Fresh and Cryopreserved Human...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Fresh and Cryopreserved Human...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Fresh and Cryopreserved Human...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Troubles respiratoires › Syndrome de détresse respiratoire de l'adulte › Fresh and Cryopreserved Human...
questionsmedicales.fr › Structures de l'embryon › Foetus › Cordon ombilical › Fresh and Cryopreserved Human...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Maladies pulmonaires › Lésion pulmonaire induite par la ventilation mécanique › Fresh and Cryopreserved Human...