Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands.
DNA methylation
G-quadruplex
G4 ligand
biosensor
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
06 Dec 2021
06 Dec 2021
Historique:
received:
29
10
2021
revised:
30
11
2021
accepted:
02
12
2021
entrez:
10
12
2021
pubmed:
11
12
2021
medline:
17
12
2021
Statut:
epublish
Résumé
Genomic DNA methylation is involved in many diseases and is expected to be a specific biomarker for even the pre-symptomatic diagnosis of many diseases. Thus, a rapid and inexpensive detection method is required for disease diagnosis. We have previously reported that cytosine methylation in G-quadruplex (G4)-forming oligonucleotides develops different G4 topologies. In this study, we developed a method for detecting CpG methylation in G4-forming oligonucleotides based on the structural differences between methylated and unmethylated G4 DNAs. The differences in G4 topologies due to CpG methylation can be discriminated by G4 ligands. We performed a binding assay between methylated or unmethylated G4 DNAs and G4 ligands. The binding abilities of fluorescent G4 ligands to
Identifiants
pubmed: 34884964
pii: ijms222313159
doi: 10.3390/ijms222313159
pmc: PMC8658440
pii:
doi:
Substances chimiques
BCL2 protein, human
0
Ligands
0
Proto-Oncogene Proteins c-bcl-2
0
VEGFA protein, human
0
Vascular Endothelial Growth Factor A
0
DNA
9007-49-2
HRAS protein, human
EC 3.6.5.2
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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