Preclinical Investigation of Radiopharmaceuticals: An Accurate and Multidisciplinary Approach.
Radiopharmaceutical
[
autoradiography
biodistribution
micro-PET
pre-clinical studies
Journal
Current radiopharmaceuticals
ISSN: 1874-4729
Titre abrégé: Curr Radiopharm
Pays: United Arab Emirates
ID NLM: 101468718
Informations de publication
Date de publication:
2022
2022
Historique:
received:
02
08
2021
revised:
14
10
2021
accepted:
01
11
2021
pubmed:
11
12
2021
medline:
18
5
2022
entrez:
10
12
2021
Statut:
ppublish
Résumé
The development of less expensive and pivotal methodologies capable of supporting the researchers in the radiopharmaceutical pre-clinical investigations could provide a crucial incentive for conducting biomedical research involved in the realization of tailored target therapies. The aim of this pilot study was to evaluate the capability of a digital autoradiography system equipped with a laser scanning device to perform [ PC3 prostate cancer cells were used to develop NOD/SCID mice xenografts. The biodistribution of the radiopharmaceuticals was evaluated at 30, 60, and 120 min after injecting in excised organs by using a digital autoradiography system equipped with a super-resolution laser screen. Histological and immunohistochemical analyses were performed to correlate the [ The reported data clearly indicate the possibility of performing accurate biodistribution studies using the digital autoradiographic system equipped with a super-resolution screen. Specifically, a significant increase in the [ The use of an autoradiography system in pre-clinical investigations could shed new light on the molecular mechanisms that orchestrate the tissues damage induced by therapeutical radiopharmaceuticals.
Sections du résumé
BACKGROUND
BACKGROUND
The development of less expensive and pivotal methodologies capable of supporting the researchers in the radiopharmaceutical pre-clinical investigations could provide a crucial incentive for conducting biomedical research involved in the realization of tailored target therapies.
OBJECTIVE
OBJECTIVE
The aim of this pilot study was to evaluate the capability of a digital autoradiography system equipped with a laser scanning device to perform [
METHODS
METHODS
PC3 prostate cancer cells were used to develop NOD/SCID mice xenografts. The biodistribution of the radiopharmaceuticals was evaluated at 30, 60, and 120 min after injecting in excised organs by using a digital autoradiography system equipped with a super-resolution laser screen. Histological and immunohistochemical analyses were performed to correlate the [
RESULTS
RESULTS
The reported data clearly indicate the possibility of performing accurate biodistribution studies using the digital autoradiographic system equipped with a super-resolution screen. Specifically, a significant increase in the [
CONCLUSION
CONCLUSIONS
The use of an autoradiography system in pre-clinical investigations could shed new light on the molecular mechanisms that orchestrate the tissues damage induced by therapeutical radiopharmaceuticals.
Identifiants
pubmed: 34886790
pii: CRP-EPUB-119323
doi: 10.2174/1874471014666211209154317
doi:
Substances chimiques
Fluorine Radioisotopes
0
Radiopharmaceuticals
0
Choline
N91BDP6H0X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-163Informations de copyright
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