Proteomic analysis of serum in workers exposed to diesel engine exhaust.
SOMAscan
carcinogenesis
diesel engine exhaust
elemental carbon
lung cancer
proteomics
Journal
Environmental and molecular mutagenesis
ISSN: 1098-2280
Titre abrégé: Environ Mol Mutagen
Pays: United States
ID NLM: 8800109
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
25
11
2021
received:
16
08
2021
accepted:
09
12
2021
pubmed:
12
12
2021
medline:
6
5
2022
entrez:
11
12
2021
Statut:
ppublish
Résumé
Diesel engine exhaust (DEE) is classified as a Group 1 human carcinogen. Using a targeted proteomics approach, we aimed to identify proteins associated with DEE and characterize these markers to understand the mechanisms of DEE-induced carcinogenicity. In this cross-sectional molecular epidemiology study, we measured elemental carbon (EC) using a personal air monitor and quantified 1317 targeted proteins in the serum using the SOMAScan assay (SOMALogic) among 19 diesel exposed factory workers and 19 unexposed controls. We used linear regressions to identify proteins associated with DEE and examined their exposure-response relationship across levels of EC using linear trend tests. We further examined pathway enrichment of DEE-related proteins using MetaCore. Occupational exposure to DEE was associated with altered levels of 22 serum proteins (permutation p < .01). Of these, 13 proteins (CXCL11, HAPLN1, FLT4, CD40LG, PES1, IGHE.IGK..IGL, TNFSF9, PGD, NAGK, CCL25, CCL4L1, PDXK, and PLA2G1B) showed an exposure-response relationship with EC (p trend < .01), with serum levels of all but PLA2G1B declining with increasing air levels of EC. For instance, C-X-C Motif Chemokine Ligand 11 (CXCL11) showed the most significant association with DEE (β = -0.25; permutation p = .00004), where mean serum levels were 4121.1, 2356.7, and 2298.8 relative fluorescent units among the unexposed, lower exposed (median, range : 56.9, 40.2-62.1 μg/m
Substances chimiques
Air Pollutants, Occupational
0
PES1 protein, human
0
RNA-Binding Proteins
0
Vehicle Emissions
0
Carbon
7440-44-0
Group IB Phospholipases A2
EC 3.1.1.4
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-28Informations de copyright
© 2021 Environmental Mutagen Society. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
Références
Balkwill, F. & Mantovani, A. (2001) Inflammation and cancer: back to Virchow? Lancet, 357(9255), 539-545.
Bassig, B.A., Dai, Y., Vermeulen, R., Ren, D., Hu, W., Duan, H. et al. (2017) Occupational exposure to diesel engine exhaust and alterations in immune/inflammatory markers: a cross-sectional molecular epidemiology study in China. Carcinogenesis, 38(11), 1104-1111.
Benjamini, Y., Drai, D., Elmer, G., Kafkafi, N. & Golani, I. (2001) Controlling the false discovery rate in behavior genetics research. Behavioural Brain Research, 125(1-2), 279-284.
Birch M.E. (2003) Monitoring of diesel particulate exhaust in the workplace. NIOSH manual of analytical methods (NMAM). p 154.
Bracke, K.R., Demedts, I.K., Joos, G.F. & Brusselle, G.G. (2007) CC-chemokine receptors in chronic obstructive pulmonary disease. Inflammation & Allergy Drug Targets, 6(2), 75-79.
Candia, J., Cheung, F., Kotliarov, Y., Fantoni, G., Sellers, B., Griesman, T. et al. (2017) Assessment of variability in the SOMAscan assay. Scientific Reports, 7(1), 14248.
Chiang, L.L., Chen, H.C., Lee, C.N., Chuang, K.J., Chen, T.T., Yeh, C.T. et al. (2013) Serum protein oxidation by diesel exhaust particles: effects on oxidative stress and inflammatory response in vitro. Chemico-Biological Interactions, 206(2), 385-393.
Choi, W.-I., Park, S.H., Park, B.J. & Lee, C.W. (2018) Interstitial lung disease and lung cancer development: a 5-year nationwide population-based study. Cancer Research and Treatment, 50(2), 374-381.
Costello, S., Attfield, M.D., Lubin, J.H., Neophytou, A.M., Blair, A., Brown, D.M. et al. (2018) Ischemic heart disease mortality and diesel exhaust and respirable dust exposure in the diesel exhaust in miners study. American Journal of Epidemiology, 187(12), 2623-2632.
Dai, Y., Ren, D., Bassig, B.A., Vermeulen, R., Hu, W., Niu, Y. et al. (2018) Occupational exposure to diesel engine exhaust and serum cytokine levels. Environmental and Molecular Mutagenesis, 59(2), 144-150.
Demedts, I.K., Bracke, K.R., Van Pottelberge, G., Testelmans, D., Verleden, G.M., Vermassen, F.E. et al. (2007) Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine, 175(10), 998-1005.
Dostert, C., Grusdat, M., Letellier, E. & Brenner, D. (2019) The TNF family of ligands and receptors: communication modules in the immune system and beyond. Physiological Reviews, 99(1), 115-160.
Drizik, E., Corbett, S., Zheng, Y., Vermeulen, R., Dai, Y., Hu, W. et al. (2020) Transcriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure. Environment International, 137, 105506.
Durham, A.L. & Adcock, I.M. (2015) The relationship between COPD and lung cancer. Lung Cancer, 90(2), 121-127.
Ferguson, J.M., Costello, S., Elser, H., Neophytou, A.M., Picciotto, S., Silverman, D.T. et al. (2020) Chronic obstructive pulmonary disease mortality: the diesel exhaust in miners study (DEMS). Environmental Research, 180, 108876.
Gomes, M., Teixeira, A.L., Coelho, A., Araújo, A. & Medeiros, R. (2014) The role of inflammation in lung cancer. Advances in Experimental Medicine and Biology, 816, 1-23.
Good, P.I. (1994) Permutation tests: a practical guide to resampling methods for testing hypotheses. Berlin: Springer-Verlag.
Hanahan, D. & Weinberg, R.A. (2011) Hallmarks of cancer: the next generation. Cell, 144(5), 646-674.
Health Council of the Netherlands. (2019) Diesel engine exhaust: health-based recommended occupational exposure limit. The Hague.
Hernán, M.A., Hernández-Díaz, S., Werler, M.M. & Mitchell, A.A. (2002) Causal knowledge as a prerequisite for confounding evaluation: an application to birth defects epidemiology. American Journal of Epidemiology, 155(2), 176-184.
Homey, B., Dieu-Nosjean, M.C., Wiesenborn, A., Massacrier, C., Pin, J.J., Oldham, E. et al. (2000) Up-regulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis. Journal of Immunology, 164(12), 6621-6632.
Hooven, L.A. & Baird, W.M. (2008) Proteomic analysis of MCF-7 cells treated with benzo[a]pyrene, dibenzo[a,l]pyrene, coal tar extract, and diesel exhaust extract. Toxicology, 249(1), 1-10.
International Agency for Research on Cancer. (2014) Diesel and gasoline engine exhausts and some nitroarenes. Lyon, France: International Agency for Research on Cancer.
Jaguin, M., Fardel, O. & Lecureur, V. (2015) Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2. PLoS One, 10(2), e0116560.
Janatpour, M.J., Hudak, S., Sathe, M., Sedgwick, J.D. & McEvoy, L.M. (2001) Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment. The Journal of Experimental Medicine, 194(9), 1375-1384.
Jantzen, K., Roursgaard, M., Desler, C., Loft, S., Rasmussen, L.J. & Møller, P. (2012) Oxidative damage to DNA by diesel exhaust particle exposure in co-cultures of human lung epithelial cells and macrophages. Mutagenesis, 27(6), 693-701.
Kim, C.H., Tworoger, S.S., Stampfer, M.J., Dillon, S.T., Gu, X., Sawyer, S.J. et al. (2018) Stability and reproducibility of proteomic profiles measured with an aptamer-based platform. Scientific Reports, 8(1), 8382.
Koutros, S., Kogevinas, M., Friesen, M.C., Stewart, P.A., Baris, D., Karagas, M.R. et al. (2020) Diesel exhaust and bladder cancer risk by pathologic stage and grade subtypes. Environment International, 135, 105346.
Lan, Q., Vermeulen, R., Dai, Y., Ren, D., Hu, W., Duan, H. et al. (2015) Occupational exposure to diesel engine exhaust and alterations in lymphocyte subsets. Occupational and Environmental Medicine, 72(5), 354-359.
Le parlement Europeen et le conseil de l'union Europeenne. (2019) DIRECTIVE (UE) 2019/130 DU PARLEMENT EUROPÉEN ET DU CONSEIL. Journal Officiel de l'Union Européenne, 30, 112-120.
Lewis, J.A., Rao, K.M., Castranova, V., Vallyathan, V., Dennis, W.E. & Knechtges, P.L. (2007) Proteomic analysis of bronchoalveolar lavage fluid: effect of acute exposure to diesel exhaust particles in rats. Environmental Health Perspectives, 115(5), 756-763.
Li, J., Zhuang, Q., Lan, X., Zeng, G., Jiang, X. & Huang, Z. (2013) PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer. IUBMB Life, 65(12), 1017-1025.
Lim, R.J., Liu, B., Krysan, K. & Dubinett, S.M. (2020) Lung cancer and immunity markers. Cancer Epidemiology, Biomarkers & Prevention, 29(12), 2423-2430.
Naccache, J.-M., Gibiot, Q., Monnet, I., Antoine, M., Wislez, M., Chouaid, C. et al. (2018) Lung cancer and interstitial lung disease: a literature review. Journal of Thoracic Disease, 10(6), 3829-3844.
National Institute for Occupational Safety and Health. (2003) NIOSH manual of analytical methods (NMAM). Ashley K OCP: DHHS (NIOSH).
Noll, J., Gilles, S., Wu, H.W. & Rubinstein, E. (2015) The relationship between elemental carbon and diesel particulate matter in underground metal/nonmetal mines in the United States and coal mines in Australia. Journal of Occupational and Environmental Hygiene, 12(3), 205-211.
Pedeli, X., Hoek, G. & Katsouyanni, K. (2011) Risk assessment of diesel exhaust and lung cancer: combining human and animal studies after adjustment for biases in epidemiological studies. Environmental Health, 10(1), 30.
Peters, A., von Klot, S., Heier, M., Trentinaglia, I., Hörmann, A., Wichmann, H.E. et al. (2004) Exposure to traffic and the onset of myocardial infarction. The New England Journal of Medicine, 351(17), 1721-1730.
Pine, S.R., Mechanic, L.E., Enewold, L., Chaturvedi, A.K., Katki, H.A., Zheng, Y.L. et al. (2011) Increased levels of circulating interleukin 6, interleukin 8, C-reactive protein, and risk of lung cancer. Journal of the National Cancer Institute, 103(14), 1112-1122.
Riedl, M. & Diaz-Sanchez, D. (2005) Biology of diesel exhaust effects on respiratory function. The Journal of Allergy and Clinical Immunology, 115(2), 221-228.
Rohloff, J.C., Gelinas, A.D., Jarvis, T.C., Ochsner, U.A., Schneider, D.J., Gold, L. et al. (2014) Nucleic acid ligands with protein-like side chains: modified aptamers and their use as diagnostic and therapeutic agents. Molecular Therapy: Nucleic Acids, 3, e201.
Sekine, Y., Katsura, H., Koh, E., Hiroshima, K. & Fujisawa, T. (2012) Early detection of COPD is important for lung cancer surveillance. European Respiratory Journal, 39(5), 1230-1240.
Shannon, P., Markiel, A., Ozier, O., Baliga, N.S., Wang, J.T., Ramage, D. et al. (2003) Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Research, 13(11), 2498-2504.
Shiels, M.S., Chaturvedi, A.K., Katki, H.A., Gochuico, B.R., Caporaso, N.E. & Engels, E.A. (2011) Circulating markers of interstitial lung disease and subsequent risk of lung cancer. Cancer Epidemiology, Biomarkers & Prevention, 20(10), 2262-2272.
Shiels, M.S., Pfeiffer, R.M., Hildesheim, A., Engels, E.A., Kemp, T.J., Park, J.H. et al. (2013) Circulating inflammation markers and prospective risk for lung cancer. Journal of the National Cancer Institute, 105(24), 1871-1880.
Shiels, M.S., Shu, X.-O., Chaturvedi, A.K., Gao, Y.-T., Xiang, Y.-B., Cai, Q. et al. (2017) A prospective study of immune and inflammation markers and risk of lung cancer among female never smokers in Shanghai. Carcinogenesis, 38(10), 1004-1010.
Silverman, D.T., Samanic, C.M., Lubin, J.H., Blair, A.E., Stewart, P.A., Vermeulen, R. et al. (2012) The diesel exhaust in miners study: a nested case-control study of lung cancer and diesel exhaust. Journal of the National Cancer Institute, 104(11), 855-868.
Vermeulen, R., Portengen, L., Silverman, D.T., Garshick, E. & Steenland, K. (2014) Meta-analysis of lung cancer risk from exposure to diesel exhaust: Vermeulen et al. respond. Environmental Health Perspectives, 122(9), A230-A231.
Wacholder, S., Chanock, S., Garcia-Closas, M., El Ghormli, L. & Rothman, N. (2004) Assessing the probability that a positive report is false: an approach for molecular epidemiology studies. Journal of the National Cancer Institute, 96(6), 434-442.
Wang, J., Sun, J., Zhang, N., Yang, R., Li, H., Zhang, Y. et al. (2019) PES1 enhances proliferation and tumorigenesis in hepatocellular carcinoma via the PI3K/AKT pathway. Life Sciences, 219, 182-189.
Xiao, G.G., Wang, M., Li, N., Loo, J.A. & Nel, A.E. (2003) Use of proteomics to demonstrate a hierarchical oxidative stress response to diesel exhaust particle chemicals in a macrophage cell line. The Journal of Biological Chemistry, 278(50), 50781-50790.
Yang, H.M., Antonini, J.M., Barger, M.W., Butterworth, L., Roberts, B.R., Ma, J.K. et al. (2001) Diesel exhaust particles suppress macrophage function and slow the pulmonary clearance of listeria monocytogenes in rats. Environmental Health Perspectives, 109(5), 515-521.