Investigation of serum levels of orexin-A, transforming growth factor β, and leptin in patients with multiple sclerosis.


Journal

Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384

Informations de publication

Date de publication:
Jan 2022
Historique:
revised: 26 11 2021
received: 23 10 2021
accepted: 29 11 2021
pubmed: 12 12 2021
medline: 19 3 2022
entrez: 11 12 2021
Statut: ppublish

Résumé

Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease affecting various inflammatory and nutritional parameters. Therefore, this study aimed to investigate the relationship between the Body Mass Index (BMI) of MS patients and the serum levels of leptin, orexin-A, and Transforming Growth Factor β (TGF-β). This cross-sectional study included 25 patients suffering from MS and 40 healthy individuals as the case and control groups, respectively. The serum levels of leptin, orexin-A, and TGF-β were assessed in the participants using the Enzyme-Linked Immunosorbent Assay methods. Moreover, data were analyzed using the descriptive statistical indices, t-test, chi-square test, and linear regression test. According to our results, the participants' mean age was 38.04 ± 7.53 and 40.23 ± 5.88 in the case and control groups, respectively. Also, the groups were not significantly different in gender, age, alcohol consumption, and smoking (p > 0.05). It was found that the mean serum levels of orexin-A and TGF-β were significantly lower in the MS patients compared to the control group, while the mean serum leptin levels were significantly higher (42.8 vs. 18.9 ng/ml, p < 0.001). Moreover, there was no significant relationship between the BMI of the MS patients and their serum levels of orexin-A, TGF-β, and leptin (p > 0.05). In conclusion, we found significantly lower levels of orexin-A and TGF-β and a significantly higher level of leptin in the MS patients compared to the control group. In addition, there was no significant relationship between the BMI and the serum levels of orexin-A, TGF-β, and leptin in MS patients.

Sections du résumé

BACKGROUND BACKGROUND
Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease affecting various inflammatory and nutritional parameters. Therefore, this study aimed to investigate the relationship between the Body Mass Index (BMI) of MS patients and the serum levels of leptin, orexin-A, and Transforming Growth Factor β (TGF-β).
METHODS METHODS
This cross-sectional study included 25 patients suffering from MS and 40 healthy individuals as the case and control groups, respectively. The serum levels of leptin, orexin-A, and TGF-β were assessed in the participants using the Enzyme-Linked Immunosorbent Assay methods. Moreover, data were analyzed using the descriptive statistical indices, t-test, chi-square test, and linear regression test.
RESULTS RESULTS
According to our results, the participants' mean age was 38.04 ± 7.53 and 40.23 ± 5.88 in the case and control groups, respectively. Also, the groups were not significantly different in gender, age, alcohol consumption, and smoking (p > 0.05). It was found that the mean serum levels of orexin-A and TGF-β were significantly lower in the MS patients compared to the control group, while the mean serum leptin levels were significantly higher (42.8 vs. 18.9 ng/ml, p < 0.001). Moreover, there was no significant relationship between the BMI of the MS patients and their serum levels of orexin-A, TGF-β, and leptin (p > 0.05).
CONCLUSIONS CONCLUSIONS
In conclusion, we found significantly lower levels of orexin-A and TGF-β and a significantly higher level of leptin in the MS patients compared to the control group. In addition, there was no significant relationship between the BMI and the serum levels of orexin-A, TGF-β, and leptin in MS patients.

Identifiants

pubmed: 34894407
doi: 10.1002/jcla.24170
pmc: PMC8761413
doi:

Substances chimiques

LEP protein, human 0
Leptin 0
Lipids 0
Orexins 0
Transforming Growth Factor beta 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e24170

Subventions

Organisme : Tabriz University of Medical Sciences (ID.: IR T.B.Z.M.E.D.REC.1398.527)

Informations de copyright

© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

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Auteurs

Sepideh Moharami (S)

Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Alireza Nourazarian (A)

Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, Iran.

Masoud Nikanfar (M)

Department of Neurology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Delara Laghousi (D)

Social Determinant of Health Research Center, Health Management and Safety Promotion Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.

Behrouz Shademan (B)

Department of Medical Biology, Faculty of Medicine, EGE University, Izmir, Turkey.

Omid Joodi Khanghah (O)

Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Fatemeh Khaki-Khatibi (F)

Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

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