Impacts of lipopolysaccharide on fetal lung developmental maturity and surfactant protein B and surfactant protein C protein expression in gestational diabetes mellitus rats.
Animals
Body Weight
Case-Control Studies
Diabetes, Gestational
/ blood
Disease Models, Animal
Female
Gene Expression Regulation, Developmental
/ drug effects
Lipopolysaccharides
/ adverse effects
Lung
/ drug effects
Male
Peptides
/ genetics
Pregnancy
Pulmonary Surfactant-Associated Protein B
/ genetics
Random Allocation
Rats
Information technology
fetal lung developmental maturity
gdm rats
sp-b and sp-c protein expression
Journal
Bioengineered
ISSN: 2165-5987
Titre abrégé: Bioengineered
Pays: United States
ID NLM: 101581063
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
pubmed:
14
12
2021
medline:
24
2
2022
entrez:
13
12
2021
Statut:
ppublish
Résumé
The rise of bioinformatics based on computer medicine provides a new method to reveal the complex biological data. This experiment is to explore the impacts of lipopolysaccharide on fetal lung developmental maturity and expressions of lung surfactant protein B (SP-B) and lung surfactant protein C (SP-C) in rats with gestational diabetes mellitus (GDM), thereby discussing the mechanism of developmental disorders in rats. Forty-eight conceived female rats were experimental subjects. Twenty-eight rats were randomly selected to construct the GDM models. All conceived rats underwent section on the 21st day of pregnancy. The ultrastructure of alveolar type II epithelial cells and the morphology of lung tissue were observed under a microscope. The protein localization and expression of SP-B and SP-C were determined by immunohistochemistry; the protein levels of SP-B and SP-C were determined by Western blot. Blood glucose and body weight of the GDM group were higher than those of the control group; the number of alveoli and alveolar area in the GDM group was lower than those in the control group; the alveolar interval in the GDM group was significantly higher than that in the control group (
Identifiants
pubmed: 34898355
doi: 10.1080/21655979.2021.2013099
pmc: PMC8805987
doi:
Substances chimiques
Lipopolysaccharides
0
Peptides
0
Pulmonary Surfactant-Associated Protein B
0
Sftpc protein, rat
133135-52-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
834-843Références
Anesth Analg. 2017 Aug;125(2):491-498
pubmed: 28277329
BMC Anesthesiol. 2020 Jan 9;20(1):14
pubmed: 31918675
J Perinatol. 2016 Feb;36(2):90-4
pubmed: 26562371
Pediatr Rev. 2014 Oct;35(10):417-28; quiz 429
pubmed: 25274969
Pediatr Infect Dis J. 2016 Oct;35(10):1161-2
pubmed: 27314825
Nutrients. 2018 Jan 31;10(2):
pubmed: 29385062
Taiwan J Obstet Gynecol. 2018 Aug;57(4):487-492
pubmed: 30122566
PLoS One. 2018 Apr 20;13(4):e0195609
pubmed: 29677194
Exp Ther Med. 2018 Aug;16(2):1397-1402
pubmed: 30116389
Intensive Care Med. 2017 Oct;43(10):1453-1463
pubmed: 28762180
Eur Rev Med Pharmacol Sci. 2017 Dec;21(24):5702-5710
pubmed: 29272006
S Afr Med J. 2017 May 24;107(6):523-527
pubmed: 28604326
FASEB J. 2015 Oct;29(10):4236-47
pubmed: 26089319
PLoS One. 2017 Jun 15;12(6):e0178022
pubmed: 28617807