Replication protein A is required for juvenile hormone-dependent vitellogenesis and oocyte maturation in locusts.
DNA replication
Fat body
Insulin
Juvenile hormone
Vitellogenin
Journal
Journal of insect physiology
ISSN: 1879-1611
Titre abrégé: J Insect Physiol
Pays: England
ID NLM: 2985080R
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
12
10
2021
revised:
05
11
2021
accepted:
06
12
2021
pubmed:
14
12
2021
medline:
27
1
2022
entrez:
13
12
2021
Statut:
ppublish
Résumé
Aside from inhibiting insect metamorphosis, juvenile hormone (JH) has a well-known role in stimulating various aspects of insect reproduction. Replication protein A (RPA), a heterotrimeric complex comprised of RPA1, RPA2 and RPA3 subunits plays an essential role in DNA replication and DNA repair. Here we report that RPAs are highly expressed in the fat body of adult female locust, Locusta migratoria. While RPA1 is upregulated by the JH receptor Methoprene-tolerant (Met), RPA2 and RPA3 expression appears to be primarily controlled by Forkhead box O transcription factor (FoxO). Knockdown of RPA1, RPA2 or RPA3 results in markedly reducd vitellogenin (Vg) expression in the fat body, accompanied by arrested ovarian growth and inhibited oocyte maturation. In addition, depletion of an RPA subunit leads to increased expression of other RPA subunits as well as a pro-apoptotic gene, Smac that is involved in DNA repair and apoptosis. The data indicate a crucial role of RPAs in JH-dependent vitellogenesis and oocyte maturation.
Identifiants
pubmed: 34902432
pii: S0022-1910(21)00155-4
doi: 10.1016/j.jinsphys.2021.104345
pii:
doi:
Substances chimiques
Juvenile Hormones
0
Replication Protein A
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104345Informations de copyright
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