The utilization of Blaptica dubia cockroaches as an in vivo model to test antibiotic efficacy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 12 2021
14 12 2021
Historique:
received:
13
07
2021
accepted:
03
12
2021
entrez:
15
12
2021
pubmed:
16
12
2021
medline:
28
1
2022
Statut:
epublish
Résumé
Insects are now well recognized as biologically relevant alternative hosts for dozens of mammalian pathogens and they are routinely used in microbial pathogenesis studies. Unfortunately, these models have yet to be incorporated into the drug development pipeline. The purpose of this work was to begin to evaluate the utility of orange spotted (Blaptica dubia) cockroaches in early antibiotic characterization. To determine whether these model hosts could exhibit mortality when infected with bacteria that are pathogenic to humans, we subjected B. dubia roaches to a range of infectious doses of Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii to identify the medial lethal dose. These results showed that lethal disease did not develop following infection of high doses of S. aureus, and A. baumannii. However, cockroaches infected with E. coli and K. pneumoniae succumbed to infection (LD50s of 5.82 × 10
Identifiants
pubmed: 34907348
doi: 10.1038/s41598-021-03486-3
pii: 10.1038/s41598-021-03486-3
pmc: PMC8671488
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
24004Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103434
Pays : United States
Organisme : NHLBI NIH HHS
ID : R15 HL147135
Pays : United States
Informations de copyright
© 2021. The Author(s).
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