Methylene blue elicits non-genotoxic H
DNA damage
Methylene blue
Neuroprotection
Rotenone
Toxicity
mtDNA
Journal
Journal of applied biomedicine
ISSN: 1214-0287
Titre abrégé: J Appl Biomed
Pays: Poland
ID NLM: 101221755
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
10
01
2018
accepted:
14
05
2019
entrez:
15
12
2021
pubmed:
1
6
2019
medline:
1
6
2019
Statut:
ppublish
Résumé
Methylene blue (MB) is a promising compound with a broad range of neuroprotective activity. One of therapeutic effects is the activation of mitochondrial biogenesis via Nrf2/ARE signaling cascade. Probably, mild oxidative stress caused by MB-depended H2O2 production is a trigger for activation of this signaling cascade. So mechanistically, MB can be regarded as prooxidant. We investigated the dose-dependent H2O2 production in intact brain mitochondria and showed the increase in the H2O2 production after adding as little as 50 nM MB. We have not found genotoxic effect of therapeutic concentration of MB to mitochondrial genome. 100 μM MB selectively damaged fragments of mitochondrial DNA, which correlated with the number of purine-T-G-purine (RTGR)-sequences in studied fragments. Furthermore, 20 μM MB combined with the red light caused the formation of singlet oxygen, which strongly damaged mitochondrial DNA in all studied fragments. We did not observe mitochondrial DNA lesions in brain after single intraperitoneal injection of MB in the concentration of 50 mg/kg. Furthermore, we showed the neuroprotective properties of MB pretreatments after rotenone injection. Therefore, we suggest that MB-induced mild oxidative stress does not have genotoxic effect on mitochondrial DNA.
Identifiants
pubmed: 34907732
doi: 10.32725/jab.2019.008
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-114Subventions
Organisme : President of the Russian Federation
ID : MK3173.2019.11
Pays : Russian Federation
Organisme : Ministry of Education and Science of the Russian Federation
ID : 6.4656.2017/8.9
Pays : Russian Federation
Organisme : Ministry of Education and Science of the Russian Federation
ID : 4656.2017/8.9
Pays : Russian Federation
Organisme : Russian Fund for Basic Research
ID : 19-44-360011
Pays : Russian Federation
Déclaration de conflit d'intérêts
The authors confirm that the study causes no conflict of interests as related to funding, commercial activity, intellectual property rights, or other conceivable issues.
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