Mass spectrometry analysis of the photosystem II assembly factor Psb27 revealed variations in its lipid modification.
Cyanobacteria
Lipoprotein
Mass spectrometry
Photosynthesis
Psb27
Journal
Photosynthesis research
ISSN: 1573-5079
Titre abrégé: Photosynth Res
Pays: Netherlands
ID NLM: 100954728
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
31
10
2021
accepted:
03
12
2021
pubmed:
16
12
2021
medline:
14
9
2022
entrez:
15
12
2021
Statut:
ppublish
Résumé
The assembly of large, multi-cofactor membrane protein complexes like photosystem II (PSII) requires a high level of coordination. The process is facilitated by a large network of auxiliary proteins that bind transiently to unassembled subunits, preassembled modules or intermediate states of PSII, which are comprised of a subset of subunits. However, analysis of these immature, partially assembled PSII complexes is hampered by their low abundance and intrinsic instability. In this study, PSII was purified from the thermophilic cyanobacterium Thermosynechococcus elongatus via Twin-Strep-tagged CP43 and further separated by ion exchange chromatography into mature and immature complexes. Mass spectrometry analysis of the immature Psb27-PSII intermediate revealed six different Psb27 proteoforms with distinct lipid modifications. The maturation and functional role of thylakoid localized lipoproteins are discussed.
Identifiants
pubmed: 34910272
doi: 10.1007/s11120-021-00891-7
pii: 10.1007/s11120-021-00891-7
pmc: PMC9458691
doi:
Substances chimiques
Bacterial Proteins
0
Lipids
0
Photosystem II Protein Complex
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
305-316Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : NO 836/3-1
Informations de copyright
© 2021. The Author(s).
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