E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori.
Bm14-3-3ζ
Bombyx mori
E2F transcription factors
E2F4
cell cycle
insect
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
14
11
2021
received:
19
08
2021
accepted:
24
11
2021
pubmed:
17
12
2021
medline:
22
7
2022
entrez:
16
12
2021
Statut:
ppublish
Résumé
The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions. Although E2Fs have been widely studied in mammals, there have been few studies performed in insects. Here, we determined the function of E2F4 in the silkworm, Bombyx mori. We demonstrate that E2F proteins are highly conserved among species from lower animals to higher mammals. Overexpression of the BmE2F4 gene led to cell cycle arrest in the G1 phase, whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S phase. These results indicate that BmE2F4 is important in cell cycle regulation. We also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN-SWU1 cells and DNA synthesis in the silk gland. Furthermore, we identified a protein called Bm14-3-3ζ that can interact with BmE2F4 and allow it to localize in the nucleus. Overexpression of the Bm14-3-3ζ gene led to cell cycle arrest in the G1 phase, while knocking down the gene increased the proportion of cells in S phase. These findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.
Identifiants
pubmed: 34913261
doi: 10.1111/1744-7917.12991
doi:
Substances chimiques
Insect Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1006-1016Informations de copyright
© 2021 Institute of Zoology, Chinese Academy of Sciences.
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