Risk Factors Associated with Recurrent Pregnancy Loss and Outcome of Pre-Implantation Genetic Screening of Affected Couples.
Array-CGH
Chromosomal Abnormalities
Recurrent Pregnancy Loss
Journal
International journal of fertility & sterility
ISSN: 2008-076X
Titre abrégé: Int J Fertil Steril
Pays: Iran
ID NLM: 101487941
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
05
10
2020
accepted:
30
01
2021
entrez:
16
12
2021
pubmed:
17
12
2021
medline:
17
12
2021
Statut:
ppublish
Résumé
Recurrent pregnancy loss (RPL) is a multifactorial disorder which affects up to 5% of couples around the world. Several factors are considered to be involved in RPL; but, the etiology remains unexplained in 35-60% of cases. The aim of this study was to assess the frequency of risk factors associated with RPL in a group of our clinic clients, and their pre-implantation genetic screening (PGS) outcome. We designed a retrospective descriptive study among, 602 Iranian couples referred to the Royan Reproductive Clinic (Tehran-Iran) from 2006 to 2018. Their karyotyping test and PGS outcomes were analyzed. PGS had been applied by array comparative genomic hybridization (array-CGH) on embryos from these patients. Also, karyotyping test had been performed using standard cytogenetic techniques. G-banding analysis revealed a frequency of 15.61% chromosomal abnormalities in RPL couples. Also, the reciprocal translocations were more frequent (33/1204 cases) compared to the other structural abnormalities. Pregnancy rate per embryo transferred were 50% with array-CGH approach. Our findings could confirm a positive correlation between chromosomal abnormalities and RPL rate. Applying PGS for the RPL couples, leads to improvement of pregnancy success rate.
Sections du résumé
BACKGROUND
BACKGROUND
Recurrent pregnancy loss (RPL) is a multifactorial disorder which affects up to 5% of couples around the world. Several factors are considered to be involved in RPL; but, the etiology remains unexplained in 35-60% of cases. The aim of this study was to assess the frequency of risk factors associated with RPL in a group of our clinic clients, and their pre-implantation genetic screening (PGS) outcome.
MATERIALS AND METHODS
METHODS
We designed a retrospective descriptive study among, 602 Iranian couples referred to the Royan Reproductive Clinic (Tehran-Iran) from 2006 to 2018. Their karyotyping test and PGS outcomes were analyzed. PGS had been applied by array comparative genomic hybridization (array-CGH) on embryos from these patients. Also, karyotyping test had been performed using standard cytogenetic techniques.
RESULTS
RESULTS
G-banding analysis revealed a frequency of 15.61% chromosomal abnormalities in RPL couples. Also, the reciprocal translocations were more frequent (33/1204 cases) compared to the other structural abnormalities. Pregnancy rate per embryo transferred were 50% with array-CGH approach.
CONCLUSION
CONCLUSIONS
Our findings could confirm a positive correlation between chromosomal abnormalities and RPL rate. Applying PGS for the RPL couples, leads to improvement of pregnancy success rate.
Identifiants
pubmed: 34913295
doi: 10.22074/IJFS.2021.137626.1027
pmc: PMC8530214
doi:
Types de publication
Journal Article
Langues
eng
Pagination
269-274Informations de copyright
Copyright© by Royan Institute. All rights reserved.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
J Hum Reprod Sci. 2018 Oct-Dec;11(4):337-342
pubmed: 30787517
J Int Med Res. 2020 Sep;48(9):300060520957820
pubmed: 32957812
Fertil Steril. 2010 Mar 1;93(4):1234-43
pubmed: 19338986
Fertil Steril. 2017 Jan;107(1):150-159.e2
pubmed: 27842992
Hum Reprod. 1990 Jul;5(5):519-28
pubmed: 2203803
Fertil Steril. 1996 Jul;66(1):24-9
pubmed: 8752606
Gynecol Endocrinol. 2016 Oct;32(sup2):1-4
pubmed: 27759447
J Obstet Gynaecol India. 2020 Jun;70(3):189-194
pubmed: 32476764
Ginekol Pol. 2016;87(10):722-726
pubmed: 27958626
Cell J. 2021 Jan;22(4):467-475
pubmed: 32347040
Reprod Biol Endocrinol. 2018 Dec 28;16(1):124
pubmed: 30593268
J Assist Reprod Genet. 2018 Mar;35(3):355-366
pubmed: 29313278
J Hum Genet. 2020 Sep;65(9):783-795
pubmed: 32398760
J Hum Reprod Sci. 2016 Apr-Jun;9(2):94-100
pubmed: 27382234
Open Access Maced J Med Sci. 2019 Dec 20;7(24):4427-4431
pubmed: 32215107
Reprod Biomed Online. 2009 Mar;18(3):424-9
pubmed: 19298745
Chin Med J (Engl). 2015 Oct 20;128(20):2772-6
pubmed: 26481744
Hum Reprod Open. 2018 Apr 06;2018(2):hoy004
pubmed: 31486805
Am J Obstet Gynecol. 1984 Jan 15;148(2):140-6
pubmed: 6691389
Rev Obstet Gynecol. 2009 Spring;2(2):76-83
pubmed: 19609401
Int J Womens Health. 2017 May 17;9:331-345
pubmed: 28553146
J Assist Reprod Genet. 2019 Dec;36(12):2557-2561
pubmed: 31734857