Differences in the sequence of PlcR transcriptional regulator-binding site affect sphingomyelinase production in Bacillus cereus.
PlcR box
phosphatidylcholine-specific phospholipase C
sphingomyelinase
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
revised:
08
12
2021
received:
28
10
2021
accepted:
12
12
2021
pubmed:
17
12
2021
medline:
6
4
2022
entrez:
16
12
2021
Statut:
ppublish
Résumé
Bacillus cereus is an opportunistic pathogen that often causes severe infections such as bacteremia, with sphingomyelinase (SMase) being a crucial virulence factor. Although many strains of B. cereus carry the SMase gene, they are classified as SMase-producing and nonproducing strains. The reason for different SMase production among B. cereus strains remains unknown. In this study, we investigated the relationship between SMase and the PlcR transcriptional regulation system to clarify the mechanism leading to varied SMase production among B. cereus strains. We analyzed the sequence of the PlcR box, which is a transcriptional regulator-binding site, located at the promoter region of SMase and phosphatidylcholine-specific phospholipase C. Based on differences in the PlcR box sequences, we classified the B. cereus strains into three groups (I, II, and III). SMase expression and activity were hardly detected in Group III strains. In Group I strains, SMase activity and its expression were maximal at the onset of the stationary phase and decreased during the stationary phase, whereas those were maintained during the stationary phase in Group II stains. On injection of B. cereus strains into mice or incubation with macrophages for phagocytosis assay, the SMase-producing Group I and II strains showed higher pathogenicity than Group III strains. These findings suggest that PlcR box sequence in B. cereus affects the production of SMase, which may provide important clinical information for the detection of highly pathogenic B. cereus strains.
Identifiants
pubmed: 34914844
doi: 10.1111/1348-0421.12959
doi:
Substances chimiques
Bacterial Proteins
0
Trans-Activators
0
Sphingomyelin Phosphodiesterase
EC 3.1.4.12
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-165Informations de copyright
© 2022 The Societies and John Wiley & Sons Australia, Ltd.
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