Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation.
Ameloblastoma
/ complications
Animals
Bone Resorption
/ etiology
Core Binding Factor Alpha 1 Subunit
/ genetics
Gene Expression
Humans
Jaw Neoplasms
/ complications
Matrix Metalloproteinase 2
/ genetics
Neoplasm Invasiveness
Osteoblasts
/ physiology
Osteogenesis
RANK Ligand
/ genetics
Rats
Stromal Cells
Tissue Engineering
/ methods
Tumor Cells, Cultured
Tumor Microenvironment
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 12 2021
16 12 2021
Historique:
received:
08
07
2021
accepted:
03
12
2021
entrez:
17
12
2021
pubmed:
18
12
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Ameloblastoma is a benign, epithelial cancer of the jawbone, which causes bone resorption and disfigurement to patients affected. The interaction of ameloblastoma with its tumour stroma drives invasion and progression. We used stiff collagen matrices to engineer active bone forming stroma, to probe the interaction of ameloblastoma with its native tumour bone microenvironment. This bone-stroma was assessed by nano-CT, transmission electron microscopy (TEM), Raman spectroscopy and gene analysis. Furthermore, we investigated gene correlation between bone forming 3D bone stroma and ameloblastoma introduced 3D bone stroma. Ameloblastoma cells increased expression of MMP-2 and -9 and RANK temporally in 3D compared to 2D. Our 3D biomimetic model formed bone nodules of an average surface area of 0.1 mm
Identifiants
pubmed: 34916549
doi: 10.1038/s41598-021-03484-5
pii: 10.1038/s41598-021-03484-5
pmc: PMC8677805
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
RANK Ligand
0
Runx2 protein, rat
0
Matrix Metalloproteinase 2
EC 3.4.24.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
24088Informations de copyright
© 2021. The Author(s).
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