Quantitative Analysis of the Extracellular Matrix by Immunoblot.
Collagen
Dot blot
ECM
FFPE
IHC
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
17
12
2021
pubmed:
18
12
2021
medline:
28
1
2022
Statut:
ppublish
Résumé
Knowing the global and local concentration of extracellular matrix proteins provides critical information for building tissue engineered constructs of healthy and diseased tissue. Here we describe a method of integrating quantitative dot blot along with immunohistochemistry on the same patient sample in order to calculate the overall protein concentration as well as the concentration in a region of interest.
Identifiants
pubmed: 34918290
doi: 10.1007/978-1-0716-1956-8_7
doi:
Substances chimiques
Extracellular Matrix Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-132Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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