Culturing Primary Human Mesothelial Cells.
Culture
Human omentum
Mesothelial cells
Ovarian cancer
Primary mesothelial cells
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
17
12
2021
pubmed:
18
12
2021
medline:
28
1
2022
Statut:
ppublish
Résumé
Mesothelial cells line the serosal cavities and associated organs. In order to metastasize to distant organs, ovarian tumor cells must first attach and then clear the mesothelial cells. Therefore, human primary mesothelial cells (HPMCs) are necessary to effectively study ovarian cancer metastases. Here, we describe methods to obtain HPMCs from human omentum and to culture in vitro.
Identifiants
pubmed: 34918292
doi: 10.1007/978-1-0716-1956-8_9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
147-154Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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