Serological differentiation of West Nile virus- and Usutu virus-induced antibodies by envelope proteins with modified cross-reactive epitopes.
Usutu virus
West Nile virus
envelope protein
fusion loop
mutations
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
01
11
2021
received:
13
09
2021
accepted:
06
12
2021
pubmed:
18
12
2021
medline:
30
9
2022
entrez:
17
12
2021
Statut:
ppublish
Résumé
West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne viruses that belong to the Japanese encephalitis virus serocomplex within the genus Flavivirus. Due to climate change and the expansion of mosquito vectors, flaviviruses are becoming endemic in increasing numbers of countries. WNV infections are reported with symptoms ranging from mild fever to severe neuro-invasive disease. Until now, only a few USUV infections have been reported in humans, mostly with mild symptoms. The serological diagnosis and differentiation between flavivirus infections, in general, and between WNV and USUV, in particular, are challenging due to the high degree of cross-reacting antibodies, especially of those directed against the conserved fusion loop (FL) domain of the envelope (E) protein. We have previously shown that E proteins containing four amino-acid mutations in and near the FL strongly reduce the binding of cross-reactive antibodies leading to diagnostic technologies with improved specificities. Here, we expanded the technology to USUV and analyzed the differentiation of USUV- and WNV-induced antibodies in humans. IgG ELISAs modified by an additional competition step with the heterologous antigen resulted in overall specificities of 93.94% for WNV Equad and 92.75% for USUV Equad. IgM antibodies against WNV could be differentiated from USUV IgM in a direct comparison using both antigens. The data indicate the potential of the system to diagnose antigenically closely related flavivirus infections.
Substances chimiques
Antibodies, Viral
0
Antigens, Heterophile
0
Epitopes
0
Immunoglobulin G
0
Immunoglobulin M
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2779-2787Subventions
Organisme : the European Union's Horizon 2020
ID : 871029
Organisme : the European Union's Horizon 2020
ID : 874735
Organisme : European Commission
Informations de copyright
© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH.
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