Comparison of Fluorometric and UV Spectrophotometric Findings for DNA Isolated From Formalin-Fixed Paraffin-Embedded Blocks, Fine Needle Aspiration Cytology Smears, and Blood.
blood
dna
ffpe
fluorometer
fnac
uv spectrophotometer
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
accepted:
13
11
2021
entrez:
20
12
2021
pubmed:
21
12
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Fine needle aspiration cytology (FNAC) smear may serve as a convenient sample for DNA extraction for molecular pathology in addition to more commonly used formalin-fixed paraffin-embedded (FFPE) sections. DNA quantification done by fluorometer is more accurate than UV vis spectrophotometer regardless of the source. This study was conducted to compare DNA yield and quality from cytology smears, FFPE sections and peripheral blood using both fluorometer and spectrophotometer. Further, introspection was made to check for the adequacy of DNA extracted from cytology smears with respect to DNA extracted from core biopsies. DNA was extracted from 10 fresh peripheral blood samples, core biopsies and FNAC smears. The DNA was quantified using a fluorimeter and UV vis spectrophotometer in all cases. Statistically significant difference was seen between the data obtained from UV vis spectrophotometry and flourometry. The quantity of DNA extracted from FNAC smears was higher than that of core biopsy as per fluorometry data (mean DNA of core biopsy = 1.9ng/µl, of FNAC = 3.3ng/µl). DNA estimation by fluorometry is more accurate and precise than spectrophotometry in FFPE, FNAC and whole blood samples. DNA yield from FNAC slides is comparable to that from core biopsies.
Identifiants
pubmed: 34926054
doi: 10.7759/cureus.19583
pmc: PMC8672151
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e19583Informations de copyright
Copyright © 2021, Paul et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Biochem Biophys Res Commun. 1985 Jul 16;130(1):118-26
pubmed: 2992457
Cancer Cytopathol. 2017 Nov;125(11):838-847
pubmed: 29024530
Br J Cancer. 1998;77(1):125-30
pubmed: 9459157
Clin Chem. 2013 May;59(5):815-23
pubmed: 23403697
PLoS One. 2017 Sep 19;12(9):e0184692
pubmed: 28926588
Cancers (Basel). 2015 Aug 28;7(3):1699-715
pubmed: 26343728
Contemp Oncol (Pozn). 2019;23(1):52-58
pubmed: 31061638
PLoS One. 2016 Mar 03;11(3):e0150528
pubmed: 26937682
J Histochem Cytochem. 2006 Mar;54(3):301-10
pubmed: 16116034
Asian Pac J Cancer Prev. 2007 Jan-Mar;8(1):55-9
pubmed: 17477772
Diagn Mol Pathol. 2013 Mar;22(1):59-64
pubmed: 23370428
J Clin Lab Anal. 1995;9(6):387-91
pubmed: 8587007
Am J Clin Pathol. 2011 Oct;136(4):564-71
pubmed: 21917678
J Clin Diagn Res. 2016 Sep;10(9):BC01-BC03
pubmed: 27790419