Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis.


Journal

Disease models & mechanisms
ISSN: 1754-8411
Titre abrégé: Dis Model Mech
Pays: England
ID NLM: 101483332

Informations de publication

Date de publication:
01 03 2022
Historique:
received: 25 06 2020
accepted: 24 11 2021
pubmed: 21 12 2021
medline: 3 5 2022
entrez: 20 12 2021
Statut: ppublish

Résumé

In the glomerulus, Bowman's space is formed by a continuum of glomerular epithelial cells. In focal segmental glomerulosclerosis (FSGS), glomeruli show segmental scarring, a result of activated parietal epithelial cells (PECs) invading the glomerular tuft. The segmental scars interrupt the epithelial continuum. However, non-sclerotic segments seem to be preserved even in glomeruli with advanced lesions. We studied the histology of the segmental pattern in Munich Wistar Frömter rats, a model for secondary FSGS. Our results showed that matrix layers lined with PECs cover the sclerotic lesions. These PECs formed contacts with podocytes of the uninvolved tuft segments, restoring the epithelial continuum. Formed Bowman's spaces were still connected to the tubular system. In biopsies of patients with secondary FSGS, we also detected matrix layers formed by PECs, separating the uninvolved from the sclerotic glomerular segments. PECs have a major role in the formation of glomerulosclerosis; we show here that in FSGS they also restore the glomerular epithelial cell continuum that surrounds Bowman's space. This process may be beneficial and indispensable for glomerular filtration in the uninvolved segments of sclerotic glomeruli.

Identifiants

pubmed: 34927672
pii: 273803
doi: 10.1242/dmm.046342
pmc: PMC8938403
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022. Published by The Company of Biologists Ltd.

Déclaration de conflit d'intérêts

Competing interests The authors declare no competing or financial interests.

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Auteurs

Laura Miesen (L)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Péter Bándi (P)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Brigith Willemsen (B)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Fieke Mooren (F)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Thiago Strieder (T)

Division of Nephrology and Immunology, University Hospital of the RWTH Aachen, 52074 Aachen, Germany.

Eva Boldrini (E)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Vedran Drenic (V)

NIPOKA GmbH, 17489 Greifswald, Germany.

Jennifer Eymael (J)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Roy Wetzels (R)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Johannes Lotz (J)

Fraunhofer Institute for Digital Medicine MEVIS, 23562 Lübeck, Germany.

Nick Weiss (N)

Fraunhofer Institute for Digital Medicine MEVIS, 23562 Lübeck, Germany.

Eric Steenbergen (E)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Toin H van Kuppevelt (TH)

Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500HB Nijmegen, the Netherlands.

Merijn van Erp (M)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Jeroen van der Laak (J)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Nicole Endlich (N)

NIPOKA GmbH, 17489 Greifswald, Germany.
Department of Anatomy and Cell Biology, University Medicine Greifswald, 17489 Greifswald, Germany.

Marcus J Moeller (MJ)

Division of Nephrology and Immunology, University Hospital of the RWTH Aachen, 52074 Aachen, Germany.

Jack F M Wetzels (JFM)

Department of Nephrology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

Jitske Jansen (J)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.
Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, 6500HB Nijmegen, The Netherlands.

Bart Smeets (B)

Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud Institute for Health Sciences, Radboud University Medical Center, 6500HB Nijmegen, The Netherlands.

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