In Situ Manipulation and Micromechanical Characterization of Diatom Frustule Constituents Using Focused Ion Beam Scanning Electron Microscopy.
biosilica
diatom frustule
hybrid materials
in situ deformation
micromanipulation
Journal
Small methods
ISSN: 2366-9608
Titre abrégé: Small Methods
Pays: Germany
ID NLM: 101724536
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
revised:
17
09
2021
received:
13
06
2021
entrez:
20
12
2021
pubmed:
21
12
2021
medline:
31
3
2022
Statut:
ppublish
Résumé
Biocomposite structures are difficult to characterize by bulk approaches due to their morphological complexity and compositional heterogeneity. Therefore, a versatile method is required to assess, for example, the mechanical properties of geometrically simple parts of biocomposites at the relevant length scales. Here, it is demonstrated how a combination of Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) and micromanipulators can be used to isolate, transfer, and determine the mechanical properties of frustule constituents of diatom Thalassiosira pseudonana (T.p.). Specifically, two parts of the diatom frustule, girdle bands and valves, are separated by FIB milling and manipulated using a sharp tungsten tip without compromising their physical or chemical integrity. In situ mechanical studies on isolated girdle bands combined with Finite Element Method (FEM) simulations, enables the quantitative assessment of the Young's modulus of this biosilica; E = 40.0 GPa. In addition, the mechanical strength of isolated valves could be measured by transferring and mounting them on top of premilled holes in the sample support. This approach may be extended to any hierarchical biocomposite material, regardless of its chemical composition, to isolate, transfer, and investigate the mechanical properties of selected constituents or specific regions.
Identifiants
pubmed: 34928031
doi: 10.1002/smtd.202100638
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100638Subventions
Organisme : Netherlands Organization for Scientific Research
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Informations de copyright
© 2021 The Authors. Small Methods published by Wiley-VCH GmbH.
Références
W. Al Zoubi, M. P. Kamil, S. Fatimah, N. Nisa, Y. G. Ko, Prog. Mater. Sci. 2020, 112, 100663.
H. B. Yao, H. Y. Fang, Z. H. Tan, L. H. Wu, S. H. Yu, Angew. Chem. 2010, 122, 2186.
R. F. Gibson, Compos. Struct. 2010, 92, 2793.
A. Ali, A. Andriyana, RSC Adv. 2020, 10, 16390.
Y.-Y. Kim, J. D. Carloni, B. Demarchi, D. Sparks, D. G. Reid, M. E. Kunitake, C. C. Tang, M. J. Duer, C. L. Freeman, B. Pokroy, Nat. Mater. 2016, 15, 903.
A. Arakaki, K. Shimizu, M. Oda, T. Sakamoto, T. Nishimura, T. Kato, Org. Biomol. Chem. 2015, 13, 974.
G. M. Luz, J. F. Mano, Compos. Sci. Technol. 2010, 70, 1777.
B. Ji, H. Gao, J. Mech. Phys. Solids 2004, 52, 1963.
F. Libonati, M. J. Buehler, Adv. Eng. Mater. 2017, 19, 1600787.
A. Ghazlan, T. Ngo, P. Tan, Y. M. Xie, P. Tran, M. Donough, Composites, Part B 2020, 205, 108513.
J. E. Rim, P. Zavattieri, A. Juster, H. D. Espinosa, J. Mech. Behav. Biomed. Mater. 2011, 4, 190.
M. Grossman, D. Pivovarov, F. Bouville, C. Dransfeld, K. Masania, A. R. Studart, Adv. Funct. Mater. 2019, 29, 1806800.
E. Munch, M. E. Launey, D. H. Alsem, E. Saiz, A. P. Tomsia, R. O. Ritchie, Science 2008, 322, 1516.
H. D. Espinosa, J. E. Rim, F. Barthelat, M. J. Buehler, Prog. Mater. Sci. 2009, 54, 1059.
B. Ji, H. Gao, Annu. Rev. Mater. Res. 2010, 40, 77.
M. A. Meyers, P.-Y. Chen, A. Y.-M. Lin, Y. Seki, Prog. Mater. Sci. 2008, 53, 1.
P.-Y. Chen, A. Lin, Y.-S. Lin, Y. Seki, A. Stokes, J. Peyras, E. Olevsky, M. A. Meyers, J. McKittrick, J. Mech. Behav. Biomed. Mater. 2008, 1, 208.
J. Wang, Q. Cheng, Z. Tang, Chem. Soc. Rev. 2012, 41, 1111.
M. Hildebrand, Chem. Rev. 2008, 108, 4855.
Y. Wang, J. Cai, Y. Jiang, X. Jiang, D. Zhang, Appl. Microbiol. Biotechnol. 2013, 97, 453.
M. Soleimani, L. Rutten, S. P. Maddala, H. Wu, E. D. Eren, B. Mezari, I. Schreur-Piet, H. Friedrich, R. A. van Benthem, Sci. Rep. 2020, 10, 19498.
N. Kröger, N. Poulsen, Annu. Rev. Genet. 2008, 42, 83.
Y. Wang, D. Zhang, J. Cai, J. Pan, M. Chen, A. Li, Y. Jiang, Appl. Microbiol. Biotechnol. 2012, 95, 1165.
J. W. Goessling, Y. Su, C. Maibohm, M. Ellegaard, M. Kühl, J. R. Soc. Interface Focus 2019, 9, 20180031.
E. Kumari, S. Görlich, N. Poulsen, N. Kröger, Adv. Funct. Mater. 2020, 30, 2000442.
C. E. Hamm, R. Merkel, O. Springer, P. Jurkojc, C. Maier, K. Prechtel, V. Smetacek, Nature 2003, 421, 841.
Z. H. Aitken, S. Luo, S. N. Reynolds, C. Thaulow, J. R. Greer, Proc. Natl. Acad. Sci. USA 2016, 113, 2017.
N. Almqvist, Y. Delamo, B. Smith, N. Thomson, Å. Bartholdson, R. Lal, M. Brzezinski, P. Hansma, J. Microsc. 2001, 202, 518.
D. Losic, K. Short, J. G. Mitchell, R. Lal, N. H. Voelcker, Langmuir 2007, 23, 5014.
S. Görlich, D. Pawolski, I. Zlotnikov, N. Kröger, Commun. Biol. 2019, 2, 245.
E. Topal, H. Rajendran, I. Zgłobicka, J. Gluch, Z. Liao, A. Clausner, K. J. Kurzydłowski, E. Zschech, Nanomaterials 2020, 10, 959.
J. Romann, M. S. Chauton, S. M. Hanetho, M. Vebner, M. Heldal, C. Thaulow, O. Vadstein, G. Tranell, M.-A. Einarsrud, J. Porous Mater. 2016, 23, 905.
C. Jiang, H. Lu, H. Zhang, Y. Shen, Y. Lu, Scanning 2017, 2017, 6215691.
M. S. Wang, I. Kaplan-Ashiri, X. L. Wei, R. Rosentsveig, H. D. Wagner, R. Tenne, L. M. Peng, Nano Res. 2008, 1, 22.
Z. Shan, Jom 2012, 64, 1229.
H. Asayesh-Ardakani, W. Yao, Y. Yuan, A. Nie, K. Amine, J. Lu, R. Shahbazian-Yassar, Small Methods 2017, 1, 1700202.
C. Shi, D. K. Luu, Q. Yang, J. Liu, J. Chen, C. Ru, S. Xie, J. Luo, J. Ge, Y. Sun, Microsyst. Nanoeng. 2016, 2, 16024.
X. Zhou, T. Li, Y. Cui, Y. Fu, Y. Liu, L. Zhu, ACS Appl. Mater. Interfaces 2019, 11, 1733.
F. Komissarenko, G. Zograf, S. Makarov, M. Petrov, I. Mukhin, Nanomaterials 2020, 10, 1306.
T. Przybilla, B. A. Zubiri, A. M. Beltrán, B. Butz, A. G. Machoke, A. Inayat, M. Distaso, W. Peukert, W. Schwieger, E. Spiecker, Small Methods 2018, 2, 1700276.
P. A. Shade, M. A. Groeber, J. C. Schuren, M. D. Uchic, Integr. Mater. Manuf. Innovation 2013, 2, 100.
N. Antoniou, K. Rykaczewski, M. D. Uchic, MRS Bull. 2014, 39, 347.
J. Zang, L. Bao, R. A. Webb, X. Li, Nano Lett. 2011, 11, 4885.
S. Romeis, J. Paul, P. Herre, D. de Ligny, J. Schmidt, W. Peukert, Scr. Mater. 2015, 108, 84.
M. Yılmaz, N. Wollschläger, M. N. Esfahani, W. Österle, Y. Leblebici, B. E. Alaca, Nanotechnology 2017, 28, 115302.
S. M. La Vars, M. R. Johnston, J. Hayles, J. R. Gascooke, M. H. Brown, S. C. Leterme, A. V. Ellis, Anal. Bioanal. Chem. 2013, 405, 3359.
M. Sumper, E. Brunner, ChemBioChem 2008, 9, 1187.
E. V. Armbrust, J. A. Berges, C. Bowler, B. R. Green, D. Martinez, N. H. Putnam, S. Zhou, A. E. Allen, K. E. Apt, M. Bechner, Science 2004, 306, 79.
M. Johnston, J. Gascooke, A. Ellis, S. Leterme, Analyst 2018, 143, 4930.
A. Bussard, E. Corre, C. Hubas, E. Duvernois-Berthet, G. Le Corguillé, L. Jourdren, F. Coulpier, P. Claquin, P. J. Lopez, Environ. Microbiol. 2017, 19, 909.
H. Wu, H. Su, R. R. Joosten, A. D. Keizer, L. S. van Hazendonk, M. J. Wirix, J. P. Patterson, J. Laven, G. de With, H. Friedrich, Small Methods 2021, 5, 2001287.
P. Schweizer, C. Dolle, D. Dasler, G. Abellán, F. Hauke, A. Hirsch, E. Spiecker, Nat. Commun. 2020, 11, 1743.
A. I. Denisyuk, F. E. Komissarenko, I. S. Mukhin, Microelectron. Eng. 2014, 121, 15.
A. I. Denisyuk, A. V. Krasavin, F. E. Komissarenko, I. S. Mukhin, Adv. Imaging Electron Phys. 2014, 186, 101.
J. Paul, S. Romeis, J. Tomas, W. Peukert, Adv. Powder Technol. 2014, 25, 136.
M. Mačković, F. Niekiel, L. Wondraczek, E. Spiecker, Acta Mater. 2014, 79, 363.
Y.-J. Hu, G. Zhao, M. Zhang, B. Bin, T. Del Rose, Q. Zhao, Q. Zu, Y. Chen, X. Sun, M. de Jong, Npj Comput. Mater. 2020, 6, 25.
P. Jimbert, M. Iturrondobeitia, R. Fernandez-Martinez, T. Guraya, J. Ibarretxe, Adv. Mater. Res. 2016, 1139, 20.