The dopamine receptor agonist apomorphine stabilizes neurotoxic α-synuclein oligomers.

Parkinson´s disease apomorphine dopamine neurotoxicity oligomer α-synuclein

Journal

FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157

Informations de publication

Date de publication:
02 2022
Historique:
revised: 13 11 2021
received: 26 10 2021
accepted: 10 12 2021
pubmed: 21 12 2021
medline: 3 3 2022
entrez: 20 12 2021
Statut: ppublish

Résumé

The misfolding and aggregation of the protein α-synuclein (aSyn) into potentially neurotoxic oligomers is believed to play a pivotal role in the neuropathogenesis of Parkinson's disease (PD). Herein, we explore how apomorphine (Apo), a nonselective dopamine D1 and D2 receptor agonist utilized in the therapy for PD, affects the aggregation and toxicity of aSyn in vitro. Our data indicated that Apo inhibits aSyn fibrillation leading to the formation of large oligomeric species (Apo-aSyn-O), which exhibit remarkable toxicity in mesencephalic dopaminergic neurons in primary cultures. Interestingly, purified Apo-aSyn-O, even at very low concentrations, seems to be capable of converting unmodified aSyn monomer into neurotoxic species. Collectively, our findings warn for a possible dangerous effect of Apo on aSyn misfolding/aggregation pathway.

Identifiants

pubmed: 34928512
doi: 10.1002/1873-3468.14263
pmc: PMC8972942
mid: NIHMS1788207
doi:

Substances chimiques

SNCA protein, human 0
alpha-Synuclein 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

309-322

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM136686
Pays : United States
Organisme : NIA NIH HHS
ID : R37 AG019391
Pays : United States

Informations de copyright

© 2021 Federation of European Biochemical Societies.

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Auteurs

Vanderlei de Araujo Lima (VA)

Department of Physical Chemistry, Federal University of Rio de Janeiro, Brazil.
Graduate Program in Chemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Rodrigo Esquinelato (R)

Department of Physical Chemistry, Federal University of Rio de Janeiro, Brazil.

Phelippe Carmo-Gonçalves (P)

Department of Physical Chemistry, Federal University of Rio de Janeiro, Brazil.
Graduate Program in Chemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Lucas Alex do Nascimento (LAD)

Department of Physical Chemistry, Federal University of Rio de Janeiro, Brazil.

Hudson Lee (H)

Department of Biochemistry, Weill Cornell Medical College, Cornell University, New York, NY, USA.

David Eliezer (D)

Department of Biochemistry, Weill Cornell Medical College, Cornell University, New York, NY, USA.

Luciana Romão (L)

Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Cristian Follmer (C)

Department of Physical Chemistry, Federal University of Rio de Janeiro, Brazil.
Graduate Program in Chemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

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Classifications MeSH